School of Public Health, Lanzhou University, Lanzhou 730000, P.R. China.
Analyst. 2021 May 21;146(10):3328-3335. doi: 10.1039/d1an00004g. Epub 2021 Apr 16.
Mycotoxins cause significant harm to human health, so it is imperative to develop a highly sensitive and easy-to-operate method for the detection of mycotoxins. Herein, a fluorescence-based magnetic separation immunoassay for simultaneous detection of mycotoxins fumonisin B1 and zearalenone is established. The method employed high fluorescent upconversion-nanoparticles(UCNPs) conjugated with biotinylated antigens as upconversion fluoroscent probes. Magnetic nanoparticles(MNPs) immobilized with monoclonal antibodies are used as immune-capture probes. Highly sensitive detection of FB1 and ZEN was achieved based on the luminescence properties of UCNPs and the separation effects of MNPs. The results showed a robust linear correlation between the enhanced fluorescence emission intensity and the logarithmic concentrations of FB1 and ZEN under the optimal conditions (R(FB1) = 0.9965, R(ZEN) = 0.9976), and the linear ranges were 0.05-5 ng mL. Furthermore, the limits of detection (LOD) were 0.016 ng mL for FB1 and 0.012 ng mL for ZEN. The standard addition method was used to determine the content of FB1 and ZEN in the samples to evaluate the accuracy of the process. The average recoveries were 89.48% to 113.69% and 85.97% to 113.82%, respectively. Compared with the other five mycotoxins, this method had high selectivity. It is expected that the multi-component simultaneous detection can be further realized by using the multicolor labeling characteristics of UCNPs.
真菌毒素对人类健康造成重大危害，因此开发高灵敏度且易于操作的真菌毒素检测方法迫在眉睫。本研究建立了一种基于荧光的磁分离免疫测定法，用于同时检测真菌毒素伏马菌素 B1 和玉米赤霉烯酮。该方法采用高荧光上转换纳米粒子（UCNPs）与生物素化抗原偶联作为上转换荧光探针。将单克隆抗体固定在磁性纳米粒子（MNPs）上作为免疫捕获探针。基于 UCNPs 的发光特性和 MNPs 的分离效果，实现了对 FB1 和 ZEN 的高灵敏度检测。结果表明，在最佳条件下（R(FB1) = 0.9965，R(ZEN) = 0.9976），增强的荧光发射强度与 FB1 和 ZEN 的对数浓度之间存在稳健的线性相关性，线性范围为 0.05-5 ng mL。此外，FB1 和 ZEN 的检出限（LOD）分别为 0.016 ng mL 和 0.012 ng mL。采用标准加入法测定样品中 FB1 和 ZEN 的含量，以评估该方法的准确性。平均回收率分别为 89.48%至 113.69%和 85.97%至 113.82%。与其他五种真菌毒素相比，该方法具有较高的选择性。预计通过利用 UCNPs 的多色标记特性，可以进一步实现多组分同时检测。
