Military Medical Sciences Academy, Academy of Military Sciences, Tianjin 300050, China.
Biosensors (Basel). 2024 Jul 21;14(7):355. doi: 10.3390/bios14070355.
Mycotoxins are secondary products produced primarily by fungi and are pathogens of animals and cereals, not only affecting agriculture and the food industry but also causing great economic losses. The development of rapid and sensitive methods for the detection of mycotoxins in food is of great significance for livelihood issues. This study employed an amino-functionalized zirconium luminescent metal-organic framework (LOF) (i.e., UiO-66-NH. Click chemistry was utilized to assemble UiO-66-NH in a controlled manner, generating LOF assemblies to serve as probes for fluorescence-linked immunoassays. The proposed fluoroimmunoassay method for Zearalenone (ZEN) and Fumonisin B1 (FB1) detection based on the UiO-66-NH assembled probe (CLICK-FLISA) afforded a linear response range of 1-20 μmol/L for ZEN, 20 μmol/L for FB1, and a very low detection limit (0.048-0.065 μmol/L for ZEN; 0.048-0.065 μmol/L for FB1). These satisfying results demonstrate promising applications for on-site quick testing in practical sample analysis. Moreover, the amino functionalization may also serve as a modification strategy to design luminescent sensors for other food contaminants.
真菌主要产生的次生代谢产物即为霉菌毒素，是动物和谷物的病原体，不仅影响农业和食品工业，还会造成巨大的经济损失。开发用于检测食品中霉菌毒素的快速、灵敏方法对于民生问题具有重要意义。本研究采用氨基功能化的锆发光金属有机骨架（LOF）（即 UiO-66-NH2）。点击化学被用于以可控的方式组装 UiO-66-NH2，生成 LOF 组装体作为荧光联免疫分析的探针。基于 UiO-66-NH2 组装探针（CLICK-FLISA）检测玉米赤霉烯酮（ZEN）和伏马菌素 B1（FB1）的荧光免疫分析方法，ZEN 的线性响应范围为 1-20 μmol/L，FB1 的线性响应范围为 20 μmol/L，检测限非常低（ZEN 为 0.048-0.065 μmol/L；FB1 为 0.048-0.065 μmol/L）。这些令人满意的结果表明，该方法有望在实际样品分析中进行现场快速检测。此外，氨基功能化也可以作为设计其他食品污染物发光传感器的修饰策略。
