Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast, UK, BT9 5DL.
BioMensio Limited, Hermiankatu 6-8H, 33720, Tampere, Finland.
Talanta. 2021 Jan 15;222:121521. doi: 10.1016/j.talanta.2020.121521. Epub 2020 Aug 26.
Mycotoxins produced by Fusarium species including trichothecenes, zearalenone and fumonisins, can co-contaminate food and feed throughout the supply chain, including cereal grains and animal feeds. There is an increasing demand to enhance global food security by improving the monitoring of mycotoxins throughout our food supply chain. For time and cost-efficient analysis, rapid tests capable of detecting multiple toxins from a single sample are ideal. Considering these current trends in mycotoxin testing, this project examined the feasibility of using both a portable and non-portable mass-based biosensor for multiplex mycotoxin detection. The biosensor was a mass sensitive microarray (MSMA) which consisted of 4 × 16 miniaturized mass sensitive transducer pixels based on solidly mounted resonator (SMR) technology. Functionalisation of individual pixels on the sensor surface using nano-spotting technology for the simultaneous and semi-quantitative detection of three regulated mycotoxins: T2-toxin (T2) zearalenone (ZEN), and fumonisin B1 (FumB1) was examined. With the integration of portable and non-portable microfluidic devices for antibody and standard sample injections, competitive inhibition assays were developed followed by singleplex and multiplex calibration curves. The characteristics and performance of the MSMA were evaluated including sensitivity which was determined as the concentration causing 50% inhibition. Sensitivity of singleplex assays using the portable microfluidic device (PMD) were 1.3 ng/ml, 2.0 ng/ml and 6.8 ng/ml for T2, FumB1 and ZEN, respectively. Sensitivity of the multiplex assay again using the PMD was 6.1 ng/ml, 3.6 ng/ml and 2.4 ng/ml for T2, FumB1 and ZEN, respectively. The PMD was an easy to use and highly sensitive screening tool which has been demonstrated for the multiplex detection of three regulated mycotoxins. Analysis was in real time and results were fully digital. Since detection of analytes was by mass it was both a label-free and cost-efficient method proposed method of analysis for mycotoxins.
镰刀菌属产生的真菌毒素，包括单端孢霉烯族化合物、玉米赤霉烯酮和伏马菌素，可在整个供应链中（包括谷物和动物饲料）与食物和饲料共同污染。为了提高全球粮食安全，人们对加强对我们食物链中真菌毒素的监测的需求日益增加。为了实现时间和成本效益分析，能够从单个样品中检测多种毒素的快速检测方法是理想的。考虑到目前真菌毒素检测的趋势，本项目研究了使用便携式和非便携式基于质量的生物传感器进行多重真菌毒素检测的可行性。该生物传感器是一种质量敏感微阵列（MSMA），由基于固态谐振器（SMR）技术的 4×16 个小型质量敏感换能器像素组成。使用纳米点样技术对传感器表面的各个像素进行功能化，用于同时和半定量检测三种受监管的真菌毒素：T2 毒素（T2）、玉米赤霉烯酮（ZEN）和伏马菌素 B1（FumB1）。通过集成便携式和非便携式微流控装置用于抗体和标准样品的注入，开发了竞争性抑制测定法，随后进行了单重和多重校准曲线。评估了 MSMA 的特性和性能，包括作为引起 50%抑制的浓度的灵敏度。使用便携式微流控装置（PMD）进行单重分析的灵敏度分别为 T2、FumB1 和 ZEN 的 1.3ng/ml、2.0ng/ml 和 6.8ng/ml。再次使用 PMD 进行多重分析的灵敏度分别为 T2、FumB1 和 ZEN 的 6.1ng/ml、3.6ng/ml 和 2.4ng/ml。PMD 是一种易于使用且高度敏感的筛选工具，已证明可用于多重检测三种受监管的真菌毒素。分析是实时进行的，结果完全数字化。由于分析物的检测是通过质量进行的，因此它既是一种无标记的、具有成本效益的真菌毒素分析方法。
