School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Cell Cycle. 2021 Jun;20(11):1033-1040. doi: 10.1080/15384101.2021.1919830. Epub 2021 May 18.
Apoptosis is closely associated with many diseases. Detection of apoptosis can be achieved by morphology, biochemistry, molecular biology, immunology, and other techniques. However, as technologies are increasingly used for the detection of apoptosis, many researchers are confused about how to choose a suitable method to detect apoptosis. Selection of a suitable detection method for apoptosis will help clinical diagnosis and prevention of diseases. This article reviews the selection of optimal apoptosis-detection methods based on research purposes and technique principles.
细胞凋亡与许多疾病密切相关。细胞凋亡的检测可通过形态学、生物化学、分子生物学、免疫学等技术实现。然而,随着技术越来越多地用于检测细胞凋亡,许多研究人员对于如何选择合适的方法来检测细胞凋亡感到困惑。选择合适的细胞凋亡检测方法将有助于临床疾病的诊断和预防。本文基于研究目的和技术原理,综述了最佳细胞凋亡检测方法的选择。