Kulikov A V, Voronova I P, Zhanaeva E Iu
Vopr Med Khim. 1988 Mar-Apr;34(2):120-3.
An assay of tryptophane hydroxylase activity in brain was based on fluorometric measurement of serotonin after condensation with o-phthalic aldehyde in 10 N HCl. The method proved to be more sensitive and simple as compared with the previously reported technique and enabled to measure the enzyme activity in brain samples as little as 10 mg. The method is recommended for laboratory routine use.
脑内色氨酸羟化酶活性的测定基于在10N盐酸中与邻苯二甲醛缩合后对血清素进行荧光测量。与先前报道的技术相比，该方法被证明更灵敏、更简单，并且能够测量低至10mg脑样本中的酶活性。推荐该方法用于实验室常规操作。
