Zhang Ya-Ting, Cai Rong-Lin, Cui Shuai, Wei Xiao-Tong, Yu Qing, Wu Li-Bin, Hu Ling
College of Acupuncture and Moxibustion, Anhui University of Chinese Medicine, Hefei 230012, China.
College of Acupuncture and Moxibustion, Anhui University of Chinese Medicine, Hefei 230012, China; Anhui Key Laboratory of Acupuncture and Moxibustion, Hefei 230038.
Zhen Ci Yan Jiu. 2021 Aug 25;46(8):671-8. doi: 10.13702/j.1000-0607.201176.
To observe the effect of electroacupuncture (EA) preconditioning of "Shenmen" (HT7)-"Tongli" (HT5) segment of the Heart Meridian on neuronal electrical activities of lateral hypothalamus area (LHA) in rats with myocardial ischemia reperfusion injury (MIRI), so as to investigate its possible mechanism underlying improvement of MIRI.
Twenty-four SD rats were randomly divided into sham, model, EA and damage + EA (D+EA) groups, with 6 rats in each group. The MIRI model was established by occlusion of the anterior descending branch (ADB) of the left coronary artery for 30 min, followed by reperfusion for 2 h. In the sham operation group, the ADB was only threaded beneath the artery without ligation. EA (2 Hz, 1 V) was applied to bilateral HT7-HT5 for 20 min, once daily for 7 days. In the D+EA group, FN was injected with 0.4 μL of 1 g/L kainic acid solution. Electrocardiogram (ECG) of the neck-thoracic lead was recorded by using PowerLab. The electrical activities of the LHA region were recorded by the implanted microelectrode array (2×4) and Plexon multi-channel acquisition system. Cluster analysis of neuronal signals was carried out by Offline Sorter software. The discharge waveforms, autocorrelation and cross-correlation of neuronal activities were analyzed by using Neuro Explorer software.
Compared with the sham group, the ECG-ST height was significantly increased (<0.05), the discharge frequency of pyramidal neurons was significantly reduced (<0.01), while the discharge frequency of interneurons was increased (<0.01) in the model group. After EA treatment, the ECG-ST height was significantly decreased (<0.05), the discharge frequency of pyramidal neurons was significantly increased (<0.01) relevant to the model group. In comparison with the EA group, the ECG-ST height in the D+EA group was significantly elevated (<0.05), whereas the discharge frequency of pyramidal neurons was significantly decreased (<0.01). The discharge frequency of interneurons was kept increasing after MIRI. Real-time spectrum analysis showed that the local field potential spectrum energy of the EA group was significantly lower than that of the model group and the D+EA group.
EA preconditioning of Heart Meridian can significantly increase the discharge frequency of LHA excitatory pyramidal neurons and reduce the energy of the local field potential spectrum, which maybe one of the important mechanisms of EA in anti -MIRI.
观察心经“神门”（HT7）-“通里”（HT5）段电针预处理对心肌缺血再灌注损伤（MIRI）大鼠下丘脑外侧区（LHA）神经元电活动的影响，探讨其改善MIRI的可能机制。
将24只SD大鼠随机分为假手术组、模型组、电针组和损伤+电针（D+EA）组，每组6只。采用结扎左冠状动脉前降支30 min，再灌注2 h的方法制备MIRI模型。假手术组仅在动脉下穿线不结扎。电针组采用2 Hz、1 V，针刺双侧HT7-HT5 20 min，每日1次，连续7 d。D+EA组在FN注射0.4 μL 1 g/L的 kainic acid溶液。使用PowerLab记录颈胸导联心电图（ECG）。采用植入式微电极阵列（2×4）和Plexon多通道采集系统记录LHA区的电活动。通过Offline Sorter软件对神经元信号进行聚类分析。使用Neuro Explorer软件分析神经元活动的放电波形、自相关和互相关。
与假手术组比较，模型组ECG-ST段抬高明显（<0.05），锥体细胞放电频率明显降低（<0.01），而中间神经元放电频率增加（<0.01）。电针治疗后，与模型组比较，ECG-ST段抬高明显降低（<0.05），锥体细胞放电频率明显增加（<0.01）。与电针组比较，D+EA组ECG-ST段抬高明显升高（<0.05），而锥体细胞放电频率明显降低（<0.01）。MIRI后中间神经元放电频率持续增加。实时频谱分析显示，电针组局部场电位频谱能量明显低于模型组和D+EA组。
心经电针预处理可显著增加LHA兴奋性锥体细胞放电频率，降低局部场电位频谱能量，这可能是电针抗MIRI的重要机制之一。
