Sakaguchi Y, Konishi N, Enoki N, Shimoyama T, Hiasa Y, Murata Y, Kagoshima T, Ishikawa H, Okada R
1st Department of Pathology, Nara Medical University, Japan.
Acta Pathol Jpn. 1988 Apr;38(4):417-24. doi: 10.1111/j.1440-1827.1988.tb02315.x.
To clarify the distribution pattern of the left bundle branch (LBB) in the human heart, the AV conduction system was studied in 13 autopsied hearts obtained from subjects aged 50 to 80 years. Vertical serial sections (7 micron) of the bundle of His and LBB were prepared and every 20th section was stained alternately with hematoxylin-eosin (HE) or by the elastica van Gieson (EVG) method and examined by light microscopy. Reconstruction was performed using a two-dimensional system in order to histologically differentiate the bundle cells from Purkinje cells. The LBB bifurcated into the anterior and posterior radiations and the cells in the septal portion were almost all Purkinje cells except in two cases showing a septal branch between the two radiations. The LBB usually branched widely from the bundle of His. An extremely anterior fascicle of the LBB was found in all cases. The distribution of the LBB at the top of the ventricular septum was divided into network and continuous types. Purkinje cells were present on both the atrial and apical sides of the two main radiations. It was suggested that these findings resulted from the fact that we morphologically differentiated the bundle cells from Purkinje cells by light microscopy.
为明确人心脏中左束支（LBB）的分布模式，对13例年龄在50至80岁受试者的尸检心脏进行了房室传导系统研究。制备了希氏束和左束支的垂直连续切片（7微米），每隔20个切片交替用苏木精-伊红（HE）染色或弹性纤维染色法（EVG）染色，并进行光学显微镜检查。使用二维系统进行重建，以便从组织学上区分束细胞和浦肯野细胞。左束支分为前分支和后分支，除两例在两个分支之间有间隔分支外，间隔部分的细胞几乎全是浦肯野细胞。左束支通常从希氏束广泛分支。在所有病例中均发现了左束支的一个极前分支。左束支在室间隔顶部的分布分为网状和连续型。在两个主要分支的心房侧和心尖侧均有浦肯野细胞。提示这些发现是由于我们通过光学显微镜从形态学上区分了束细胞和浦肯野细胞这一事实所致。
