Seidel B, Hädge D, Eichler W, Fiebig H
Bereich Tierphysiologie und Immunbiologié, Karl-Marx-Universität Leipzig.
Allerg Immunol (Leipz). 1988;34(1):43-8.
The monoclonal antibodies BL-HSC/1-3 are characterized by means of indirect radioimmunotechnique with regard to their binding pattern to a set of human proteins. The results suggest a binding specificity of the three monoclonals for A-determinants of the human secretory component. However, we could not point out a different binding pattern to the immunoglobulin-bound and the free molecule, respectively. By use of appropriate immunoassays, the monoclonal antibody BL-HSC/3 is the most suitable one for the detection of the secretory component and secretory IgA in body fluids. The protein band labeled by BL-HSC/3 in the immunoblotting analysis corresponds to a molar mass of 79,000 D according to the data known for the human secretory component.
通过间接放射免疫技术对单克隆抗体BL-HSC/1-3与一组人类蛋白质的结合模式进行了表征。结果表明这三种单克隆抗体对人类分泌成分的A决定簇具有结合特异性。然而，我们未能分别指出它们与免疫球蛋白结合分子和游离分子的不同结合模式。通过使用适当的免疫测定法，单克隆抗体BL-HSC/3是检测体液中分泌成分和分泌型IgA的最合适抗体。根据已知的人类分泌成分数据，在免疫印迹分析中被BL-HSC/3标记的蛋白带对应于79,000 D的摩尔质量。
