Seyfarth M, Brock J, Fiebig H, Edelmann J, Seidel B
Institut für Immunologie, Bereichs Medizin der Wilhelm-Pieck-Universität, Rostock.
Allerg Immunol (Leipz). 1987;33(3):147-52.
This paper describes an enzyme-immunoassay for the quantitative estimation of secretory IgA(S-IgA). As solid phase a monoclonal antibody against human secretory component was used, a polyclonal peroxidase labeled anti-IgA serum as conjugate. Thus, the method was specific for S-IgA. The test was done in microtiter plates, the upper limit was 0.5 ng S-IgA/ml. The standards of our test system ran in normal ranges, the coefficients of variation were 6.9% in the within-day-test and 12.2% in the day-to-day-test. The described test system is effective and sensitive. It is suitable for clinical use.
本文描述了一种用于定量测定分泌型IgA（S-IgA）的酶免疫测定法。使用针对人分泌成分的单克隆抗体作为固相，用多克隆过氧化物酶标记的抗IgA血清作为结合物。因此，该方法对S-IgA具有特异性。试验在微量滴定板中进行，上限为0.5 ng S-IgA/ml。我们测试系统的标准品在正常范围内，批内变异系数为6.9%，批间变异系数为12.2%。所描述的测试系统有效且灵敏。适用于临床应用。
