Centre for Biodiversity Monitoring, Zoological Research Museum Alexander Koenig, Adenauerallee 160, 53113, Bonn, Germany.
Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset, DT4 8UB, UK.
Sci Rep. 2021 May 18;11(1):10498. doi: 10.1038/s41598-021-89950-6.
Forest habitats host enormous diversity, but little is known about the seasonal turnover of arthropod species between the above- and below ground forest layers. In this study, we used metabarcoding approaches to uncover arthropod diversity in different forest types and seasons. Our study shows that metabarcoding soil eDNA and Malaise trap bulk samples can provide valuable insights into the phenology and life cycles of arthropods. We found major differences in arthropod species diversity between soil samples and Malaise traps, with only 11.8% species overlap. Higher diversity levels were found in Malaise traps in summer whereas soil samples showed a diversity peak in winter, highlighting the seasonal habitat preferences and life strategies of arthropods. We conclude that collecting time series of bulk arthropod samples and eDNA in the same locations provides a more complete picture of local arthropod diversity and turnover rates and may provide valuable information on climate induced phenological shifts for long-term monitoring.
森林生境中栖息着巨大的生物多样性，但对于地上和地下森林层之间节肢动物物种的季节性更替，我们知之甚少。在这项研究中，我们使用宏条形码方法来揭示不同森林类型和季节的节肢动物多样性。我们的研究表明，土壤 eDNA 宏条形码和粘虫诱捕器混合样本可以为节肢动物的物候和生命周期提供有价值的见解。我们发现土壤样本和粘虫诱捕器之间的节肢动物物种多样性存在重大差异，只有 11.8%的物种重叠。夏季粘虫诱捕器中的多样性水平较高，而土壤样本则在冬季达到多样性高峰，突出了节肢动物的季节性栖息地偏好和生活策略。我们得出结论，在同一地点收集批量节肢动物样本和 eDNA 的时间序列可以更全面地了解当地节肢动物的多样性和更替率，并可能为长期监测提供有关气候引起的物候变化的有价值信息。
