Wamba Brice E N, Ghosh Paramita, Mbaveng Armelle T, Bhattacharya Sayantan, Debarpan Mitra, Depanwita Saha, Saunak Mustafi Mitra, Kuete Victor, Murmu Nabendu
Department of Signal Transduction and Biogenic Amines, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700026, India.
Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
Evid Based Complement Alternat Med. 2021 Jan 18;2021:8810368. doi: 10.1155/2021/8810368. eCollection 2021.
belongs to Piperaceae family and has long been used as a traditional medicine to treat various diseases in several parts of Africa. The present study aims to investigate the effect of fruit extract (PCFE) alone and in combination with dacarbazine on metastatic melanoma cell line B16-F10 and in C57BL/6J mice. Cytotoxic effects of PCFE alone and in association with dacarbazine on B16-F10 cells were studied by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and colony formation assay. Wound healing assay, immunofluorescence staining, and western blot analysis were performed to evaluate the individual and combined effect of PCFE and dacarbazine on epithelial-mesenchymal transition (EMT). For studies, C57BL/6J mice were subcutaneously injected with B16-F10 cells (5 × 10 cells/mL), and the effect of PCFE and dacarbazine was studied on tumor development. The alteration of EMT was evaluated by targeting E-cadherin, vimentin, and CD133 in PCFE alone and in combination with dacarbazine-treated tumor tissues by western blot analysis. Phytochemical screening of PCFE reveals the presence of certain secondary metabolites. Our results showed that PCFE alone and in association with dacarbazine has a good activity in preventing B16-F10 melanoma cell progression and clonogenicity. This extract also regulated EMT. results showed that PCFE (100 mg/kg body weight) reduced tumor size in C57BL/6J mice along with the decrease in the expression of vasculogenic mimicry (VM) tubes as well as an improvement in the qualitative and quantitative expression of markers involved in EMT. Our study suggests that PCFE may be useful for managing the growth and metastasis of melanoma.
属于胡椒科，长期以来在非洲多个地区被用作治疗各种疾病的传统药物。本研究旨在研究胡椒果实提取物（PCFE）单独以及与达卡巴嗪联合对转移性黑色素瘤细胞系B16-F10和C57BL/6J小鼠的影响。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法和集落形成试验研究了PCFE单独以及与达卡巴嗪联合对B16-F10细胞的细胞毒性作用。进行伤口愈合试验、免疫荧光染色和蛋白质印迹分析以评估PCFE和达卡巴嗪对上皮-间质转化（EMT）的单独及联合作用。在体内研究中，将B16-F10细胞（5×10⁶细胞/mL）皮下注射到C57BL/6J小鼠体内，研究PCFE和达卡巴嗪对肿瘤发展的影响。通过蛋白质印迹分析靶向单独的PCFE以及与达卡巴嗪联合处理的肿瘤组织中的E-钙黏蛋白、波形蛋白和CD133来评估EMT的改变。PCFE的植物化学筛选揭示了某些次生代谢产物的存在。我们的结果表明，PCFE单独以及与达卡巴嗪联合在预防B16-F10黑色素瘤细胞进展和克隆形成方面具有良好活性。该提取物还调节了EMT。体内结果表明，PCFE（100mg/kg体重）可减小C57BL/6J小鼠的肿瘤大小，同时血管生成拟态（VM）管的表达减少，以及参与EMT的标志物的定性和定量表达得到改善。我们的研究表明，PCFE可能对控制黑色素瘤的生长和转移有用。
