Belkin M, Brown R D, Wright J G, LaMorte W W, Hobson R W
Department of Surgical Research, Boston University School of Medicine, Massachusetts.
Am J Surg. 1988 Aug;156(2):83-6. doi: 10.1016/s0002-9610(88)80360-x.
Characterization of ischemia-reperfusion injury of skeletal muscle remains poorly defined. A new quantitative assay to measure ischemic skeletal muscle injury is described and validated in a rat hindlimb model. This biochemical spectrophotometric technique measures triphenyltetrazolium chloride reduction in ischemic muscle. The reduction assay demonstrated significant injury after 3 hours of ischemia (25.4 +/- 9.7 percent of control activity; p less than 0.05). More severe injury occurred after 4 or more hours (less than 3 percent of control activity; p less than 0.05). This assay is an objective and quantitative method for characterizing ischemia-reperfusion injury.
骨骼肌缺血再灌注损伤的特征仍未明确界定。本文描述了一种用于测量缺血性骨骼肌损伤的新定量测定方法，并在大鼠后肢模型中进行了验证。这种生化分光光度技术可测量缺血肌肉中氯化三苯基四氮唑的还原情况。还原测定显示，缺血3小时后出现明显损伤（为对照活性的25.4±9.7%；p<0.05）。4小时或更长时间后出现更严重的损伤（低于对照活性的3%；p<0.05）。该测定法是一种用于表征缺血再灌注损伤的客观定量方法。
