McGregor A R, Crookall-Greening J O, Landon J, Smith D S
Clin Chim Acta. 1978 Feb 1;83(1-2):161-6. doi: 10.1016/0009-8981(78)90219-x.
A polarisation fluoroimmunoassay for the determination of phenytoin levels in serum is described. Fluoresceinthiocarbamyl alpha,alpha-diphenylglycine (FTC-DPG) is used as an easily prepared fluorescent-labelled analogue of phenytoin. Interference from the non-specific binding of FTC-DPG by serum proteins is eliminated by proteolytic degradation of samples prior to assay. The method employs stable, non-radioactive reagents, requires no separation procedure, and involves only 1.25 microliter of serum. Analyses of serum samples from patients receiving phenytoin correlate well (r = 0.96) with an established gas-liquid chromatographic method.
本文描述了一种用于测定血清中苯妥英水平的偏振荧光免疫分析法。荧光硫代氨基甲酰α,α-二苯基甘氨酸（FTC-DPG）用作苯妥英易于制备的荧光标记类似物。在测定前通过对样品进行蛋白水解降解来消除血清蛋白对FTC-DPG非特异性结合的干扰。该方法使用稳定的非放射性试剂，无需分离步骤，且仅需1.25微升血清。对接受苯妥英治疗患者的血清样本分析结果与既定的气液色谱法相关性良好（r = 0.96）。
