Kamel R S, McGregor A R, Landon J, Smith D S
Clin Chim Acta. 1978 Oct 2;89(1):93-8. doi: 10.1016/0009-8981(78)90364-9.
A separation fluoroimmunoassay system for phenytoin was established based on the use of a specific rabbit antiserum, a fluorescein-labelled ligand, and precipitation of the antibody-bound fraction of the labelled ligand with sodium sulphate. Simple measures were taken to obviate non-specific binding and matrix effects. Either the free fraction (in the supernatant) or thebound fraction of the labelled ligand was quantitated fluorimetrically. Assays of patient serum samples by either method correlated well with established gas-liquid chromatographic and radioimmunoassay techniques. Advantages of a separation based procedure as compared with previously described non-separation hapten fluoroimmunoassay techniques are that only simple instrumentation and assay reagents are required, and that the separation step may enable the removal of any interfering intrinsic fluorescence of serum samples.
基于使用特异性兔抗血清、荧光素标记配体以及用硫酸钠沉淀标记配体的抗体结合部分，建立了一种苯妥英的分离荧光免疫分析系统。采取了简单措施来消除非特异性结合和基质效应。通过荧光法对标记配体的游离部分（在上清液中）或结合部分进行定量。用这两种方法对患者血清样本进行检测，结果与既定的气液色谱法和放射免疫分析法技术相关性良好。与先前描述的非分离半抗原荧光免疫分析技术相比，基于分离的方法的优点是仅需要简单的仪器和检测试剂，并且分离步骤可以去除血清样本中任何干扰性的固有荧光。
