肠癌细胞中的内脂素与组蛋白 H3K9me 整体水平
Visfatin and global histone H3K9me levels in colon cancer.
机构信息
Department of Biochemistry, College of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Prince Sultan Military Medical City, Riyadh, Saudi Arabia.
出版信息
Ann Med. 2021 Dec;53(1):647-652. doi: 10.1080/07853890.2021.1925737.
BACKGROUND
Visfatin is considered to be a biomarker in various types of cancers, including colon cancer. Moreover, evidence for epigenetic mechanism must be reported for an association between visfatin level and colon cancer. Therefore, this study was designed to investigate the status of visfatin expression and the global histone three modifications in colon cancerous tissue.
METHODS
Colon cancerous tissue and paired adjacent non-cancerous tissue from 30 patients were used to determine the global histone three modifications using Western blot. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assess visfatin's expression level in tissues.
RESULTS
The visfatin and the global H3K9me expression levels were significantly higher in colon cancerous tissue than in the paired adjacent non-cancerous tissue.
CONCLUSION
The present study makes a crucial noteworthy contribution to visfatin effect on colon cancer development H3K9me.
背景
脂联素被认为是多种癌症（包括结肠癌）的生物标志物。此外，对于脂联素水平与结肠癌之间的关联，必须报告表观遗传机制的证据。因此，本研究旨在研究结肠癌组织中脂联素表达和组蛋白三修饰的状态。
方法
使用 Western blot 法检测 30 例患者的结肠癌组织和配对的相邻非癌组织中的组蛋白三修饰。定量实时聚合酶链反应（qRT-PCR）用于评估组织中脂联素的表达水平。
结果
结肠癌组织中脂联素和组蛋白 H3K9me 的表达水平明显高于配对的相邻非癌组织。
结论
本研究对脂联素影响结肠癌发展 H3K9me 的作用做出了重要的贡献。
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