培养条件对金黄色葡萄球菌104株葡萄球菌凝固酶产生的影响
Influence of cultivation conditions on the production of staphylocoagulase by Staphylococcus aureus 104.
作者信息
Engels W, Kamps M, van Boven C P
出版信息
J Gen Microbiol. 1978 Dec;109(2):237-43. doi: 10.1099/00221287-109-2-237.
PMID:34009
Abstract
High yields of staphylocoagulase from Staphylococcus aureus 104 were obtained in a simple salts medium supplement with glycerol, casein hydrolysate and three vitamins. Conditions of oxygen-limitation (dissolved oxygen concentration less than 2%), a pH of 7.4, a temperature of 35 degrees C and a 1 in 10 inoculum of overnight culture were required for optimal yields of staphylocoagulase.
摘要
在一种添加了甘油、酪蛋白水解物和三种维生素的简单盐培养基中，金黄色葡萄球菌104能高产葡萄球菌凝固酶。要获得葡萄球菌凝固酶的最佳产量，需要有氧气限制条件（溶解氧浓度低于2%）、pH值为7.4、温度为35摄氏度以及用过夜培养物按1:10接种。
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