一种快速直接的葡萄球菌凝固酶检测方法，该方法使用显色底物来鉴定金黄色葡萄球菌。
Rapid and direct staphylocoagulase assay that uses a chromogenic substrate for identification of Staphylococcus aureus.
作者信息
Engels W, Kamps M A, van Boven C P
出版信息
J Clin Microbiol. 1981 Nov;14(5):496-500. doi: 10.1128/jcm.14.5.496-500.1981.
Abstract
A rapid and direct assay of staphylocoagulase that uses chromogenic substrate Chromozym TH was developed for the routine identification of Staphylococcus aureus. False-positive and false-negative reactions were eliminated by measuring the activity of staphylocoagulase-prothrombin complex directly. The method was compared with three clotting assays. The results show that the new assay is specific, quantitative, and easily automated. If offers clinical laboratories a more reliable assay of staphylocoagulase and is a valuable aid for the rapid identification of S. aureus.
摘要
为常规鉴定金黄色葡萄球菌，开发了一种使用显色底物Chromozym TH的快速直接检测葡萄球菌凝固酶的方法。通过直接测量葡萄球菌凝固酶-凝血酶原复合物的活性，消除了假阳性和假阴性反应。将该方法与三种凝血检测方法进行了比较。结果表明，新检测方法具有特异性、定量性且易于自动化。它为临床实验室提供了一种更可靠的葡萄球菌凝固酶检测方法，对快速鉴定金黄色葡萄球菌具有重要帮助。
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