National Center for Genetic Engineering and Biotechnology, Pathumthani, Thailand.
Methods Mol Biol. 2021;2290:69-77. doi: 10.1007/978-1-0716-1323-8_5.
As the consequences of climate change become apparent, metabolic engineers and synthetic biologists are exploring sustainable sources for transportation fuels. The design and engineering of microorganisms to produce bio-gasoline and other biofuels from renewable feedstocks can significantly reduce dependence on fossil fuels as well as lower the emissions of greenhouse gases. A significant amount of research over the past two decades has led to the development of microbial strains for the production of advanced fuel compounds. Crucial to these efforts are robust methods to quantify the amount of the biofuel compound being produced as well as the other metabolites that might be present during fermentation. Here, we provide a protocol for the quantification of branched-chain alcohols, specifically isobutanol and isopropanol, using high-performance liquid chromatography (HPLC).
随着气候变化的后果变得明显，代谢工程师和合成生物学家正在探索可持续的交通燃料来源。通过设计和工程改造微生物，利用可再生原料生产生物汽油和其他生物燃料，可以显著降低对化石燃料的依赖，同时减少温室气体排放。在过去的二十年中，大量的研究导致了用于生产先进燃料化合物的微生物菌株的发展。这些努力的关键是要有强大的方法来定量检测生物燃料化合物的产量，以及发酵过程中可能存在的其他代谢物。在这里，我们提供了一种使用高效液相色谱（HPLC）定量检测支链醇（特别是异丁醇和异丙醇）的方法。
