Division of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, India.
Agronomy Department, IFAS, University of Florida, Gainesville, FL, USA.
Methods Mol Biol. 2021;2290:141-155. doi: 10.1007/978-1-0716-1323-8_10.
RNA interference (RNAi) is an innate cellular mechanism triggered by a double-stranded RNA (dsRNA) molecule causing selective inhibition of gene expression. Here, we demonstrated the RNAi technology for gene silencing in sugarcane for biofuel production. This chapter describes an efficient model system that established to target the caffeic acid O-methyltransferase (COMT) gene and the RNAi construct is designed and delivered through Agrobacterium mediated stable sugarcane transformation. Also, the approach for an analysis of resulting putative transgenic plants for a targeted RNAi mediated gene silencing is described.
RNA 干扰 (RNAi) 是一种由双链 RNA (dsRNA) 分子触发的先天细胞机制，导致基因表达的选择性抑制。在这里，我们展示了用于生物燃料生产的甘蔗基因沉默的 RNAi 技术。本章描述了一个有效的模型系统，该系统针对咖啡酸-O-甲基转移酶 (COMT) 基因，并通过农杆菌介导的稳定甘蔗转化设计和传递 RNAi 构建体。此外，还描述了针对靶向 RNAi 介导的基因沉默的潜在转基因植物进行分析的方法。
