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以及塞尔维亚梨果采后病原菌的种类()

and Species as Postharvest Pathogens of Pear Fruit () in Serbia.

作者信息

Stošić Stefan, Ristić Danijela, Savković Željko, Grbić Milica Ljaljević, Vukojević Jelena, Živković Svetlana

机构信息

Department of Plant Diseases, Institute for Plant Protection and Environment, 11000 Belgrade, Serbia.

University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac," 11000 Belgrade, Serbia.

出版信息

Plant Dis. 2021 Nov;105(11):3510-3521. doi: 10.1094/PDIS-01-21-0037-RE. Epub 2021 Nov 18.

Abstract

Pears are one of the oldest and the third most important fruit species grown in temperate regions. They are consumed because of their nutritional and health benefits, in fresh form or as various processed products. This article resolves the etiology of the -like mold symptoms on pear fruits in Serbia. Samples of pear fruits with blue mold and other -like mold symptoms were collected in Serbia from 2016 to 2019, from four storages. The recovered isolates were identified and characterized according to a polyphasic approach. Morphological and physiological analyses were performed on three media and five temperatures, respectively. Four loci (internal transcribed spacer, beta-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit) were used for sequencing, genetic identification, and phylogenetic analyses. The results of the identification by conventional and molecular methods were in agreement, and they revealed that the obtained isolates belong to five species: , , , , and . In a pathogenicity test, , , , and produced decay on artificially inoculated pear fruits, and induced tissue response lesions. The results of this study are the first reports of and as postharvest pear pathogens. Also, these are the first world records of , , and on fruits of European pear. Furthermore, this is the first finding of , , , , and on pear fruit in Serbia.

摘要

梨是温带地区种植历史最悠久且位列第三重要的水果品种之一。因其具有营养和健康益处,人们会以新鲜形式或各种加工产品的形式食用梨。本文解析了塞尔维亚梨果实上类似霉菌症状的病因。2016年至2019年期间,从塞尔维亚的四个储存库中采集了带有青霉和其他类似霉菌症状的梨果实样本。根据多相方法对分离得到的菌株进行鉴定和表征。分别在三种培养基和五个温度下进行形态学和生理学分析。使用四个基因座(内转录间隔区、β-微管蛋白、钙调蛋白和DNA依赖性RNA聚合酶II第二大亚基)进行测序、基因鉴定和系统发育分析。常规方法和分子方法的鉴定结果一致,结果表明所获得的分离菌株属于五个物种:[具体物种名称未给出]、[具体物种名称未给出]、[具体物种名称未给出]、[具体物种名称未给出]和[具体物种名称未给出]。在致病性试验中,[具体物种名称未给出]、[具体物种名称未给出]、[具体物种名称未给出]和[具体物种名称未给出]在人工接种的梨果实上产生腐烂,[具体物种名称未给出]诱导组织反应损伤。本研究结果是[具体物种名称未给出]和[具体物种名称未给出]作为采后梨病原菌的首次报道。此外,这些是[具体物种名称未给出]、[具体物种名称未给出]和[具体物种名称未给出]在欧洲梨果实上的首次世界纪录。此外,这是塞尔维亚梨果实上首次发现[具体物种名称未给出]、[具体物种名称未给出]、[具体物种名称未给出]、[具体物种名称未给出]和[具体物种名称未给出]。

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