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塞尔维亚出现新的采后真菌病原体。

New Postharvest Fungal Pathogen in Serbia.

机构信息

Department of Plant Diseases, Institute for Plant Protection and Environment, 11000 Belgrade, Serbia.

Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade, 11000 Belgrade, Serbia.

出版信息

Plant Dis. 2020 Mar;104(3):656-667. doi: 10.1094/PDIS-08-19-1806-RE. Epub 2020 Jan 21.

Abstract

is one of the important species of genus , which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with -like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, -tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are . In the pathogenicity assay, was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of in Serbia.

摘要

属于重要的物种,分布广泛,存在于多种不同的生境中。尽管曾从各种植物宿主中分离出该种,但它并未被视为重要的植物病原菌。从 2015 年到 2017 年,从塞尔维亚市场上收集到了带有状霉菌症状的水果和蔬菜。从榅桲、番茄和橙子果实、洋葱鳞茎和土豆块茎中分离出并鉴定了来源的菌株,并在形态学、生理学和分子水平上对其进行了特征描述。形态学和生理学检查包括观察微观形态、在六种不同培养基和五种不同温度下测试生长情况以及三种酶的产生。分子鉴定和特征描述使用了四个分子标记:内部转录间隔区、-微管蛋白、钙调蛋白和 DNA 依赖性 RNA 聚合酶 II 大亚基。形态学和分子分析的结果一致,证明所获得的分离物是。在致病性测定中,除了土豆块茎外,所有测试的种均被确认为的病原菌。这是首次报道在榅桲、番茄和橙子果实以及洋葱鳞茎上作为采后植物病原菌的,同时也是在塞尔维亚的首次记录。

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