College of Life Science, Shandong Normal University, Jinan 250014, PR China; Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, PR China.
College of Life Science, Shandong Normal University, Jinan 250014, PR China.
Gene. 2021 Jul 30;791:145722. doi: 10.1016/j.gene.2021.145722. Epub 2021 May 16.
Plant height is a fundamentally crucial agronomic trait to control crop growth and high yield cultivation. Several studies have been conducted on the understanding ofmolecular genetic bases of plant height in model plants and crops. However, the molecular mechanism underlying peanut plant height development is stilluncertain. In the present study, we created a peanut mutant library by fast neutron irradiation using peanut variety SH13 and identified a semi-dwarf mutant 1 (sdm1). At 84 DAP (days after planting), the main stem of sdm1 was only about 62% of SH13. The internode length of sdm1 hydroponic seedlings was found significantly shorter than that of SH13 at 14 DAP. In addition, the foliar spraying of exogenous IAA could partially restore the semi-dwarf phenotype of sdm1. Transcriptome data indicated that the differentially expressed genes (DEGs) between sdm1 and SH13 significantly enriched in diterpenoid biosynthesis, alpha-linolenic acid metabolism, brassinosteroid biosynthesis, tryptophan metabolism and plant hormone signal transduction. The expression trend of most of the genes involved in IAA and JA pathway showed significantly down- and up- regulation, which may be one of the key factors of the sdm1 semi-dwarf phenotype. Moreover, several transcription factorsand cell wall relatedgenes were expressed differentially between sdm1 and SH13. Conclusively, this research work not only provided important clues to unveil the molecular mechanism of peanut plant height regulation, but also presented basic materials for breeding peanut cultivars with ideal plant height.
株高是控制作物生长和高产栽培的一个基本农艺性状。已有多项研究旨在深入理解模式植物和作物的株高分子遗传基础。然而，花生株高发育的分子机制尚不清楚。本研究采用 SH13 花生品种通过快中子辐照创建了一个花生突变体库，并鉴定出一个半矮秆突变体 1（sdm1）。在 84 天（播种后天数），sdm1 的主茎只有 SH13 的约 62%。sdm1 水培幼苗的节间长度在 14 天（播种后天数）时明显短于 SH13。此外，外源 IAA 的叶面喷施可部分恢复 sdm1 的半矮秆表型。转录组数据表明，sdm1 和 SH13 之间的差异表达基因（DEGs）显著富集在二萜生物合成、α-亚麻酸代谢、油菜素内酯生物合成、色氨酸代谢和植物激素信号转导途径中。参与 IAA 和 JA 途径的大多数基因的表达趋势均呈显著下调和上调，这可能是 sdm1 半矮秆表型的关键因素之一。此外，sdm1 和 SH13 之间的几个转录因子和细胞壁相关基因的表达存在差异。综上所述，本研究工作不仅为揭示花生株高调控的分子机制提供了重要线索，而且为培育理想株高的花生品种提供了基础材料。
