School of Life Sciences, The Chinese University of Hong Kong, Sha Tin, N.T., Hong Kong.
School of Life Sciences, The Chinese University of Hong Kong, Sha Tin, N.T., Hong Kong.
Aquat Toxicol. 2021 Jul;236:105843. doi: 10.1016/j.aquatox.2021.105843. Epub 2021 Apr 24.
Octocrylene (OC) is a broad-spectrum ultraviolet-absorbing chemical used in sunscreen and other personal care products. Its health effects are a concern because it has been detected in water, fish, humans, and food chains. In vivo and in vitro investigations were performed in zebrafish (Danio rerio) larvae and a zebrafish liver cell line (ZFL), respectively, to understand the potential risks and molecular mechanisms of OC toxicity. The 96-h median lethal concentration (LC) of OC was determined to be 251.8 μM in larvae and 5.5 μM in ZFL cells. Quantitative real-time PCR (qRT-PCR) showed that OC induced the expression of genes for CYPs (CYP1A, CYP3A65), estrogen receptors (ERα, ERβ1, GPER), vitellogenin (VTG1), and sex determination (BRCA2, CYP19A, DMRT1, SOX9A), both in vitro and in vivo. A whole-transcriptome sequencing method was used to evaluate the gene expression profile of larvae exposed to OC. OC was found to mediate the biosynthesis of estrogens (such as estriol) and affect the antioxidant pathway (glutathione transferases and peroxisome). These findings clarify the toxic effects and molecular mechanisms of OC and support banning its use in cosmetics.
辛氧苯酮（OC）是一种广谱紫外线吸收化学物质，用于防晒霜和其他个人护理产品。由于它已在水中、鱼类、人类和食物链中被检测到，因此其健康影响令人担忧。分别在斑马鱼（Danio rerio）幼虫和斑马鱼肝细胞系（ZFL）中进行了体内和体外研究，以了解 OC 毒性的潜在风险和分子机制。OC 在幼虫中的 96 小时半数致死浓度（LC）为 251.8 μM，在 ZFL 细胞中的 LC 为 5.5 μM。实时定量 PCR（qRT-PCR）显示，OC 在体内和体外均诱导了 CYP（CYP1A、CYP3A65）、雌激素受体（ERα、ERβ1、GPER）、卵黄蛋白原（VTG1）和性别决定（BRCA2、CYP19A、DMRT1、SOX9A）基因的表达。使用全转录组测序方法评估了暴露于 OC 的幼虫的基因表达谱。OC 介导了雌激素（如雌三醇）的生物合成，并影响了抗氧化途径（谷胱甘肽转移酶和过氧化物酶体）。这些发现阐明了 OC 的毒性作用和分子机制，并支持禁止其在化妆品中的使用。
