Zhang Qiuya Y, Ma Xiaoyan Y, Wang Xiaochang C, Ngo Huu Hao
International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Key Lab of Northwest Water Resource, Environment and Ecology, MOE, Engineering Technology Research Center for Wastewater Treatment and Reuse, Shaanxi Province, China; Key Lab of Environmental Engineering, Shaanxi Province, Xi'an University of Architecture and Technology, No. 13, Yanta Road, Xi'an 710055, China.
International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Key Lab of Northwest Water Resource, Environment and Ecology, MOE, Engineering Technology Research Center for Wastewater Treatment and Reuse, Shaanxi Province, China; Key Lab of Environmental Engineering, Shaanxi Province, Xi'an University of Architecture and Technology, No. 13, Yanta Road, Xi'an 710055, China.
Chemosphere. 2016 Sep;159:433-441. doi: 10.1016/j.chemosphere.2016.06.037. Epub 2016 Jun 20.
In this study, zebrafish (Danio rerio) were exposed to a UV-filter-octocrylene (OCT) with elevated concentrations for 28 d. The total body accumulation of OCT in zebrafish was found to reach 2321.01 ("L" level), 31,234.80 ("M" level), and 70,593.38 ng g(-1) ("H" level) when the average OCT exposure concentration was controlled at 28.61, 505.62, and 1248.70 μg L(-1), respectively. Gross and histological observations as well as RT-qPCR analysis were conducted to determine the effects of OCT accumulation on zebrafish. After exposure, the gonad-somatic index and percentage of vitellogenic oocytes were found to increase significantly in the ovaries of female zebrafish at the H accumulation level. Significant up-regulation of esr1 and cyp19b were observed in the gonads, as well as vtg1 in the livers for both female and male zebrafish. At M and H accumulation levels, apparent down-regulation of ar was observed in the ovaries and testis of the female and male zebrafish, respectively. Although the extent of the effects on zebrafish differed at different accumulation levels, the induction of vtg1 and histological changes in the ovaries are indications of estrogenic activity and the inhibition of esr1 and ar showed antiestrogenic and antiandrogenic activity, respectively. Thus, as OCT could easily accumulate in aquatic life such as zebrafish, one of its most of concern hazards would be the disturbance of the histological development and its multiple hormonal activities.
在本研究中，斑马鱼（Danio rerio）暴露于浓度升高的紫外线过滤剂二乙氨羟苯甲酰基苯甲酸己酯（OCT）中28天。当OCT平均暴露浓度分别控制在28.61、505.62和1248.70μg L(-1)时，斑马鱼体内OCT的全身积累量分别达到2321.01（“L”水平）、31234.80（“M”水平）和70593.38 ng g(-1)（“H”水平）。进行了大体和组织学观察以及RT-qPCR分析，以确定OCT积累对斑马鱼的影响。暴露后，发现处于H积累水平的雌性斑马鱼卵巢中，性腺-体细胞指数和卵黄生成卵母细胞的百分比显著增加。在雌性和雄性斑马鱼的性腺中均观察到esr1和cyp19b的显著上调，在肝脏中vtg1也显著上调。在M和H积累水平时，分别在雌性和雄性斑马鱼的卵巢和睾丸中观察到ar明显下调。尽管在不同积累水平下对斑马鱼的影响程度不同，但vtg1的诱导和卵巢中的组织学变化表明有雌激素活性，而esr1和ar的抑制分别显示出抗雌激素和抗雄激素活性。因此，由于OCT很容易在斑马鱼等水生生物中积累，其最令人担忧的危害之一将是对组织学发育及其多种激素活性的干扰。
