番茄丛矮病毒RNA的体外翻译
In vitro translation of tomato bushy stunt virus RNA.
作者信息
Quintero A, Fritsch C, Hirth L
机构信息
Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France.
出版信息
Arch Virol. 1988;100(3-4):273-8. doi: 10.1007/BF01487690.
PMID:3401119
Abstract
In vitro translation of tomato bushy stunt (TBSV)-RNA in a rabbit reticulocyte system resulted in synthesis of five proteins P 18, P 25, P 34, P 35, and P 40. The P 40 protein was identified as the viral coat protein. Fractionation of TBSV-RNA and subsequent translation provided evidence for the existence of discrete subgenomic RNAs.
摘要
在兔网织红细胞系统中对番茄丛矮病毒（TBSV）-RNA进行体外翻译，结果合成了五种蛋白质P 18、P 25、P 34、P 35和P 40。P 40蛋白被鉴定为病毒外壳蛋白。对TBSV-RNA进行分级分离并随后进行翻译，为离散亚基因组RNA的存在提供了证据。
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