Hillman B I, Carrington J C, Morris T J
Department of Plant Pathology, University of California, Berkeley 94720.
Cell. 1987 Nov 6;51(3):427-33. doi: 10.1016/0092-8674(87)90638-6.
A symptom-modulating RNA associated with tomato bushy stunt virus (TBSV) was investigated with respect to physical and biological properties. Linear RNA of approximately 396 nucleotides was packaged in viral coat protein and was dependent on TBSV for replication. Coinoculation of the small RNA with TBSV resulted in the attenuation of TBSV-induced symptoms and depression of virus synthesis in whole plants. Nucleotide sequence analysis revealed that the symptom-modulating RNA was derived from 5', 3', and internal segments of the TBSV genome. The identification of this symptom-modulating RNA as a co-linear deletion mutant of the helper virus genome establishes it as the first definitive defective interfering RNA (DI RNA) to be identified in association with a plant virus.
对一种与番茄丛矮病毒（TBSV）相关的症状调节RNA的物理和生物学特性进行了研究。约396个核苷酸的线性RNA被包装在病毒外壳蛋白中，并且其复制依赖于TBSV。将这种小RNA与TBSV共同接种，导致TBSV诱导的症状减轻以及整株植物中病毒合成的抑制。核苷酸序列分析表明，这种症状调节RNA来源于TBSV基因组的5'、3'和内部片段。将这种症状调节RNA鉴定为辅助病毒基因组的共线性缺失突变体，使其成为首个与植物病毒相关的明确鉴定的缺陷干扰RNA（DI RNA）。
