环状RNA Circ0021205通过miR-204-5p/RAB22A轴促进胆管癌进展

Circular RNA Circ0021205 Promotes Cholangiocarcinoma Progression Through MiR-204-5p/RAB22A Axis.

作者信息

Tu Jianfei, Chen Weiqian, Zheng Liyun, Fang Shiji, Zhang Dengke, Kong Chunli, Yang Yang, Qiu Rongfang, Zhao Zhongwei, Lu Chenying, Lu Xiaojie, Ji Jiansong

机构信息

Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, Lishui Hospital of Zhejiang University/Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, China.

Clinical College of The Affiliated Central Hospital, Lishui University, Lishui, China.

出版信息

Front Cell Dev Biol. 2021 May 3;9:653207. doi: 10.3389/fcell.2021.653207. eCollection 2021.

Cholangiocarcinomas (CCA) are biliary tract tumors that are often challenging to diagnosis and treatment. Accumulated evidence reveals that circular RNAs (circRNAs) are involved in multiple cancer progression. However, the function of circRNAs in cholangiocarcinoma remains largely unclear. In this study, we found that circ_0021205 expression was up-regulated in CCA and positively correlated with tumor size and TNM stage. To further explore the role of circ_0021205 in CCA, cell functional assays were performed. The results showed that circ_0021205 promoted the proliferation, migration, and invasion of CCA cells. experiments showed that circ_0021205 inhibition reduced tumorigenesis in mice. In addition, mechanisms investigation demonstrated that circ_0021205 exerts its oncogenic function by sponging miR-204-5p to regulate the expression of RAB22A. Overall, this study revealed that circ_0021205 might serve as a potential diagnostic biomarker or therapeutic target for CCA.

胆管癌（CCA）是胆道肿瘤，其诊断和治疗往往具有挑战性。越来越多的证据表明，环状RNA（circRNA）参与多种癌症进展。然而，circRNA在胆管癌中的功能仍不清楚。在本研究中，我们发现circ_0021205在CCA中表达上调，且与肿瘤大小和TNM分期呈正相关。为进一步探究circ_0021205在CCA中的作用，我们进行了细胞功能实验。结果表明，circ_0021205促进了CCA细胞的增殖、迁移和侵袭。实验表明，抑制circ_0021205可减少小鼠肿瘤发生。此外，机制研究表明，circ_0021205通过海绵吸附miR-204-5p来调节RAB22A的表达，从而发挥其致癌功能。总体而言，本研究表明circ_0021205可能作为CCA潜在而言，本研究表明circ_0021205可能作为CCA潜在的诊断生物标志物或治疗靶点。