微小 RNA
MicroRNA.
机构信息
Division of Gastroenterology, University of Chicago Medicine, Chicago, Ill.
Division of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.
出版信息
J Allergy Clin Immunol. 2018 Apr;141(4):1202-1207. doi: 10.1016/j.jaci.2017.08.034. Epub 2017 Oct 23.
Abstract
MicroRNAs (miRNAs) are small endogenous RNAs that regulate gene-expression posttranscriptionally. MiRNA research in allergy is expanding because miRNAs are crucial regulators of gene expression and promising candidates for biomarker development. MiRNA mimics and miRNA inhibitors currently in preclinical development have shown promise as novel therapeutic agents. Multiple technological platforms have been developed for miRNA isolation, miRNA quantitation, miRNA profiling, miRNA target detection, and modulating miRNA levels in vitro and in vivo. Here we will review the major technological platforms with consideration given for the advantages and disadvantages of each platform.
摘要
微小 RNA（miRNAs）是一种小型内源性 RNA，可在后转录水平调节基因表达。 miRNA 在过敏中的研究正在扩展，因为 miRNA 是基因表达的重要调节剂，也是生物标志物开发的有前途的候选者。 miRNA 模拟物和 miRNA 抑制剂目前处于临床前开发阶段，已显示出作为新型治疗剂的潜力。已经开发出多种技术平台用于 miRNA 分离、miRNA 定量、miRNA 谱分析、miRNA 靶标检测以及在体外和体内调节 miRNA 水平。在这里，我们将回顾主要的技术平台，并考虑每个平台的优缺点。
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本文引用的文献
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