Minabe Y, Tsutsumi M, Yasui S, Kurachi M
Department of Neuropsychiatry, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
No To Shinkei. 1988 Apr;40(4):383-7.
To assess the appropriateness of hippocampal low-frequency kindling as an experimental model of epilepsy, we stimulated, bipolarly, left ventral hippocampus of 8 cats with 2 mA biphasic square wave pulses (1 msec duration) once a day. The pulse-interval was set at 300 msec. Recording electrodes were inserted in right ventral hippocampus, bilateral amygdaloid and bilateral globus pallidus. EEG was monitored before, during and after the delivery of stimulation. If the triggering of epileptic afterdischarge was observed on EEG monitoring, the delivery of stimulation was stopped immediately. Generalized convulsive seizures developed in all subjects within a mean of 26.2 days. In all seizures self-sustained epileptic afterdischarge, defined as epileptiform spikes three times the base line amplitude with a frequency greater than the stimulating pulse-interval (300 msec) in the stimulated hippocampus lead, was triggered abruptly. Therefore we could measure the number of stimulating pulses required for the triggering of epileptic afterdischarge, defined as pulse-number threshold, definitely in all cases. At the completion of kindling the pulse-number threshold was measured at 12.3 +/- 0.9 (+/- SE). After five generalized convulsions were induced we tested the stability of the pulse-number threshold and the duration of triggered epileptic afterdischarge. Both the two measures did not change statistically at the interstimulation interval from 24 hrs to 96 hrs. In addition we carried out testing with antiepileptic and antipsychotic drugs. Phenobarbital (10 mg/kg, i.p.) increased the pulse-number threshold and the after discharge duration simultaneously. Haloperidol (3 mg/kg, i.p.), oppositely, decreased the pulse number threshold and the after discharge duration.
为评估海马低频点燃作为癫痫实验模型的适宜性，我们对8只猫的左腹侧海马进行双极刺激，每天1次，采用2毫安双相方波脉冲（持续时间1毫秒）。脉冲间隔设定为300毫秒。记录电极插入右腹侧海马、双侧杏仁核和双侧苍白球。在刺激前、刺激期间和刺激后监测脑电图。如果在脑电图监测中观察到癫痫后放电的触发，则立即停止刺激。所有受试动物在平均26.2天内出现全身性惊厥发作。在所有发作中，自持续癫痫后放电，即在受刺激海马导联中定义为基线振幅三倍且频率大于刺激脉冲间隔（300毫秒）的癫痫样棘波，会突然触发。因此，我们能够在所有情况下明确测量触发癫痫后放电所需的刺激脉冲数，即脉冲数阈值。在点燃完成时，测量的脉冲数阈值为12.3±0.9（±标准误）。在诱发5次全身性惊厥后，我们测试了脉冲数阈值的稳定性和触发癫痫后放电的持续时间。在24小时至96小时的刺激间隔内，这两种测量在统计学上均未发生变化。此外，我们还使用抗癫痫和抗精神病药物进行了测试。苯巴比妥（10毫克/千克，腹腔注射）同时增加了脉冲数阈值和后放电持续时间。相反，氟哌啶醇（3毫克/千克，腹腔注射）降低了脉冲数阈值和后放电持续时间。
