桥接尺度：利用原位单细胞技术从细胞生物学到生理学。
Bridging scales: From cell biology to physiology using in situ single-cell technologies.
机构信息
Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA; Institute for Quantitative and Computational Biosciences, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA; Institute for Quantitative and Computational Biosciences, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Integrative Biology and Physiology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
出版信息
Cell Syst. 2021 May 19;12(5):388-400. doi: 10.1016/j.cels.2021.03.002.
Biological organization crosses multiple spatial scales: from molecular, cellular, to tissues and organs. The proliferation of molecular profiling technologies enables increasingly detailed cataloging of the components at each scale. However, the scarcity of spatial profiling has made it challenging to bridge across these scales. Emerging technologies based on highly multiplexed in situ profiling are paving the way to study the spatial organization of cells and tissues in greater detail. These new technologies provide the data needed to cross the scale from cell biology to physiology and identify the fundamental principles that govern tissue organization. Here, we provide an overview of these key technologies and discuss the current and future insights these powerful techniques enable.
生物组织跨越多个空间尺度
从分子、细胞到组织和器官。分子分析技术的不断发展使得对每个尺度的组成部分进行越来越详细的编目成为可能。然而，由于缺乏空间分析技术，使得跨越这些尺度的研究变得具有挑战性。基于高通量原位分析的新兴技术正在为更详细地研究细胞和组织的空间组织铺平道路。这些新技术提供了跨越细胞生物学到生理学的尺度所需的数据，并确定了控制组织组织的基本原理。在这里，我们概述了这些关键技术，并讨论了这些强大技术能够带来的当前和未来的见解。
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