Division of Nephrology, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
Nat Rev Nephrol. 2024 Oct;20(10):659-671. doi: 10.1038/s41581-024-00841-1. Epub 2024 May 8.
The ability to localize hundreds of macromolecules to discrete locations, structures and cell types in a tissue is a powerful approach to understand the cellular and spatial organization of an organ. Spatially resolved transcriptomic technologies enable mapping of transcripts at single-cell or near single-cell resolution in a multiplex manner. The rapid development of spatial transcriptomic technologies has accelerated the pace of discovery in several fields, including nephrology. Its application to preclinical models and human samples has provided spatial information about new cell types discovered by single-cell sequencing and new insights into the cell-cell interactions within neighbourhoods, and has improved our understanding of the changes that occur in response to injury. Integration of spatial transcriptomic technologies with other omics methods, such as proteomics and spatial epigenetics, will further facilitate the generation of comprehensive molecular atlases, and provide insights into the dynamic relationships of molecular components in homeostasis and disease. This Review provides an overview of current and emerging spatial transcriptomic methods, their applications and remaining challenges for the field.
将数百种生物大分子定位到组织中离散的位置、结构和细胞类型的能力，是理解器官细胞和空间组织的一种强大方法。空间分辨转录组学技术能够以多重方式在单细胞或接近单细胞分辨率的水平上对转录本进行定位。空间转录组学技术的快速发展加速了包括肾脏病学在内的多个领域的发现步伐。它在临床前模型和人类样本中的应用，为单细胞测序发现的新细胞类型提供了空间信息，并深入了解了局部细胞间的相互作用，从而增进了我们对损伤反应中发生的变化的理解。将空间转录组学技术与其他组学方法（如蛋白质组学和空间表观遗传学）相结合，将进一步促进全面分子图谱的生成，并深入了解在稳态和疾病中分子成分的动态关系。本文综述了当前和新兴的空间转录组学方法、它们的应用以及该领域的剩余挑战。
