细胞外囊泡通过与不同来源的间充质干细胞的串扰改变有效荷载。

Alteration of payload in extracellular vesicles by crosstalk with mesenchymal stem cells from different origin.

机构信息

Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 20 Ilsan- ro, Wonju, Gangwon-do, 26426, South Korea.

Research Institute of Hearing Enhancement, Wonju, Gangwon-do, 26426, South Korea.

出版信息

J Nanobiotechnology. 2021 May 20;19(1):148. doi: 10.1186/s12951-021-00890-9.

DOI:10.1186/s12951-021-00890-9

PMID:34016123

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8139033/

Abstract

BACKGROUND

The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads.

RESULTS

Here, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively.

CONCLUSIONS

In conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine.

摘要

背景

间充质干细胞（MSCs）衍生的细胞外囊泡（EVs）的应用需要定制材料来靶向疾病或细胞损伤。我们假设，尽管来自不同来源的干细胞在人类中有相似的有效负载，但 EVs 对一个受体细胞发挥不同的炎症作用。

结果

在这里，从脂肪组织、骨髓和扁桃体中提取的 MSC 与人中耳上皮细胞（HMEEC）之间的细胞间通讯释放的 EV 的有效负载显著增加了抗炎因子的水平。源自共培养培养基的 EV 在 3 小时内将 HMEEC 中的 TNF-α、COX-2、IL-1β 和 IL-6 水平降低至接近零。使用微阵列分析 miR-638 的表达，分别使用基因本体分析分析淀粉样蛋白-β A4 前体蛋白结合家族 A 成员 2 的表达。