Elisevich K, Ciriello J
Department of Physiology, University of Western Ontario, London, Canada.
Brain Res. 1988 Jun 14;452(1-2):141-8. doi: 10.1016/0006-8993(88)90018-2.
In chloralose anesthetized, paralyzed and artificially ventilated cats, the region of the paramedian reticular nucleus (PRN) was systematically explored for single units antidromically activated by electrical stimulation of histologically verified sites in the intermediate gray region of the upper thoracic cord (T2). These antidromically identified units were then tested for their orthodromic responses to electrical stimulation of ipsilateral carotid sinus nerve (CSN) and of pressor sites in the contralateral fastigial nucleus (FN). Sixty-two histologically verified single units, located predominantly in the caudal half of the ventral PRN, were antidromically activated with latencies corresponding to a mean conduction velocity of 36.4 +/- 2.1 m/s. Of these units 25 (40%) were excited orthodromically by stimulation of the CSN and/or FN: 5 to stimulation of the CSN only (mean latency, 18.3 +/- 9.9 ms), 6 to stimulation of the FN only (mean latency, 7 +/- 1.7 ms), and 14 to stimulation of both the CSN and FN (mean latencies, 12.3 +/- 2.9 ms and 8.4 +/- 1 ms, respectively). These data provide electrophysiological evidence for the existence of PRN reticulo-spinal neurons that integrate and relay cardiovascular afferent information from the CSN and FN to spinal autonomic neurons.
在使用水合氯醛麻醉、麻痹并进行人工通气的猫中，系统地探索了旁正中网状核（PRN）区域，寻找由电刺激组织学证实的上胸段脊髓（T2）中间灰质区域中的位点而产生逆向激活的单个神经元。然后测试这些经逆向鉴定的神经元对同侧颈动脉窦神经（CSN）和对侧顶核（FN）中升压位点的电刺激的顺向反应。62个经组织学证实的单个神经元主要位于腹侧PRN的尾侧半，以对应于平均传导速度36.4 +/- 2.1 m/s的潜伏期被逆向激活。在这些神经元中，25个（40%）通过刺激CSN和/或FN被顺向兴奋：5个仅对CSN刺激有反应（平均潜伏期，18.3 +/- 9.9 ms），6个仅对FN刺激有反应（平均潜伏期，7 +/- 1.7 ms），14个对CSN和FN刺激均有反应（平均潜伏期分别为12.3 +/- 2.9 ms和8.4 +/- 1 ms）。这些数据为PRN网状脊髓神经元的存在提供了电生理证据，这些神经元整合并将来自CSN和FN的心血管传入信息传递给脊髓自主神经元。
