Lin Xiao, Ye Minjun, Mo Wei, Zhao Hong
Department of Internal Medicine, Taizhou Hospital of Integrated Traditional Chinese and Western Medicine Wenling 317523, Zhejiang Province, China.
Department of Endocrinology, Taizhou Hospital of Integrated Traditional Chinese and Western Medicine Wenling 317523, Zhejiang Province, China.
Am J Transl Res. 2021 Apr 15;13(4):3198-3205. eCollection 2021.
This study aimed to investigate the effects of chronic hypoxia on the endocrine-related parameters in elderly rats with type 2 diabetes mellitus.
Sixty, 55-week-old, female SD rats were studied, among which 20 were randomly divided into a blank control group (the BCG), and 40 were intraperitoneally injected with low-dose streptozotocin (STZ) for modeling and divided into a model control group (the MCG, n=20) and a hypoxic group (the HG, n=20). The BCG and the MCG were fed routinely under normoxia, while the HG was fed in the hypoxic environment of an OxyCycler Model A84XO hypoxic chamber. After 12 weeks of intervention, the body weights, the fasting blood glucose (FBG) levels, the fasting insulin (FINS) levels, the blood lipid metabolism levels, the bone densities, the fresh weights of the femurs, the biomechanical properties of the femurs, the inflammatory factor levels, the H&E staining of the liver tissue, and the oil red O staining were compared.
The increases in weight gain and the FBG and FINS levels in the HG were lower than the corresponding levels in the MCG and were higher than the levels in the BCG (<0.05). The TC, TG, and LDL-C levels in the HG were lower than they were in the MCG and higher than they were in the BCG (<0.05). The bone density and fresh weight of the femurs at 12 weeks after the intervention in the HG were higher than they were in the MCG and lower than they were in the BCG (<0.05). The maximum stress, maximum load, fracture load, and elastic modulus in the HG were higher than they were in the MCG and lower than they were in the BCG (<0.05). The TNF-α, IL-6, PAI-1, and CRP levels in the HG were lower than they were in the MCG and higher than they were in the BCG (<0.05).
Chronic hypoxia can improve the endocrine parameters and insulin resistance, improve the insulin sensitivity and the femoral biomechanics, reduce the inflammatory factors levels, and improve the glucose and lipid metabolism levels and liver function in elderly rats with type 2 diabetes.
本研究旨在探讨慢性缺氧对老年2型糖尿病大鼠内分泌相关参数的影响。
选取60只55周龄雌性SD大鼠，其中20只随机分为空白对照组（BCG），40只腹腔注射低剂量链脲佐菌素（STZ）造模，分为模型对照组（MCG，n = 20）和缺氧组（HG，n = 20）。BCG和MCG在常氧条件下常规饲养，HG在OxyCycler Model A84XO缺氧舱的缺氧环境中饲养。干预12周后，比较各组大鼠的体重、空腹血糖（FBG）水平、空腹胰岛素（FINS）水平、血脂代谢水平、骨密度、股骨新鲜重量、股骨生物力学性能、炎症因子水平、肝组织苏木精-伊红（H&E）染色及油红O染色情况。
HG组体重增加量、FBG和FINS水平的升高幅度低于MCG组，高于BCG组（P<0.05）。HG组总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）水平低于MCG组，高于BCG组（P<
