Portilla-de Buen Eliseo, Ramirez-Contreras Juan Pablo, Chejfec-Ciociano Jonathan Matias, Lopez-Falcony Rodrigo, Garcia-Martinez David, Vazquez-Camacho Jose Gonzalo, Fuentes-Orozco Clotilde, Barbosa-Camacho Francisco Jose, Ibarrola-Peña Juan Carlos, Gonzalez-Ojeda Alejandro
Surgical Research Division, Western Biomedical Research Center, Western Medical Center, Mexican Institute of Social Security, Guadalajara, Jalisco, México.
Biomedical Research Unit 02, Specialties Hospital, Western Medical Center, Mexican Institute of Social Security, Guadalajara, Jalisco, México.
Res Rep Urol. 2021 May 14;13:257-262. doi: 10.2147/RRU.S307397. eCollection 2021.
Urethral stricture caused by fibrosis is a common medical condition, but top-line therapy for this pathology has a high recurrence rate. This study aimed to determine the efficacy of hyaluronic acid (HA) treatment in preventing the development of fibrosis in a rabbit model of urethral anastomosis.
This experimental study involved 20 rabbits. HA (0.5 mL, 25 μg/mL) was applied in the experimental group (n = 10) during an experimental urethral anastomosis, and sterile saline (0.9%) solution was applied in the control group (n = 10). Animals underwent reoperation 12 weeks later for urethral resection. Fibrosis, inflammation, and urethral diameter were measured by two blinded pathologists at the site of the anastomosis.
The amount of inflammatory infiltrate was similar in both groups. The thicknesses of the collagen fiber band were 275.9 ± 62.3 and 373.4 ± 44.3 μm in the study and control groups (p = 0.001), respectively, and the urethral lumen diameters at the anastomosis site at follow-up were 2575 ± 167 and 2382 ± 214 μm, respectively (p = 0.04).
HA treatment reduced fibrosis at the anastomosis site during this experiment; we suggest further research to corroborate its efficacy in the treatment of urethral stricture.
由纤维化引起的尿道狭窄是一种常见病症，但针对这种病理状况的一线治疗复发率很高。本研究旨在确定透明质酸（HA）治疗在兔尿道吻合模型中预防纤维化形成的疗效。
本实验研究涉及20只兔子。在实验组（n = 10）进行实验性尿道吻合时应用HA（0.5 mL，25 μg/mL），对照组（n = 10）应用无菌生理盐水（0.9%）溶液。12周后动物接受再次手术进行尿道切除。由两名不知情的病理学家在吻合部位测量纤维化、炎症和尿道直径。
两组的炎性浸润量相似。研究组和对照组胶原纤维带的厚度分别为275.9±62.3和373.4±44.3μm（p = 0.001），随访时吻合部位的尿道腔直径分别为2575±167和2382±214μm（p = 0.04）。
在本实验中，HA治疗减少了吻合部位的纤维化；我们建议进一步研究以证实其在治疗尿道狭窄方面的疗效。
