Kilinc Muhammet Fatih, Doluoglu Omer Gokhan, Eser Pinar Eylem, Yildiz Yildiray, Yazar Veli Mert, Ayyildiz Ali, Hucumenoglu Sema
Department of Urology, Medical Science University, Ankara Training and Research Hospital, Ankara, Turkey.
Department of Urology, Medical Science University, Ankara Training and Research Hospital, Ankara, Turkey.
Urology. 2019 Jan;123:297.e9-297.e14. doi: 10.1016/j.urology.2018.05.045. Epub 2018 Jul 20.
To determine the effects of intraurethral erythropoietin (EPO) on an experimentally induced urethral injury in a rat model with respect to wound healing enhancement and the prevention of spongiofibrosis MATERIAL AND METHODS: A urethral injury model was created by traumatizing the urethra of male rats with a tilted-tip insulin injector. Thirty rats were randomly separated into 3 groups of 10; Group 1 (control) received 0.9% saline solution twice a day, Group II received EPO 25 IU/kg once a day and 0.9% saline solution once a day, and Group III received EPO 25 IU/kg twice a day. All applications were made intraurethrally via a 24 ga catheter sheath. To investigate inflammation and spongiofibrosis and congestion of vessels in the lamina propria, the penises of the rats were harvested for histopathologic evaluation after a follow-up period of 14 days.
Histopathologic analysis revealed less fibrosis and inflammation and higher congestion of vessels in Group III that had received high-dose EPO. There was a significant decrease in both spongiofibrosis and inflammation and an increase in congestion in Groups II and III compared to the control group (P = .001, for all). In the comparison of Group II with Group III, no statistically significant differences were found in terms of these 3 parameters (P = .5, P = .6, P = .27, respectively).
The results of this study have shown that EPO has a preventive effect on spongiofibrosis and improve urethral wound healing in a rat model of urethral injury.
在大鼠模型中，确定尿道内注射促红细胞生成素（EPO）对实验性诱导的尿道损伤在促进伤口愈合和预防海绵体纤维化方面的作用。
用倾斜尖端的胰岛素注射器损伤雄性大鼠尿道，建立尿道损伤模型。30只大鼠随机分为3组，每组10只；第1组（对照组）每天两次给予0.9%生理盐水，第2组每天一次给予25 IU/kg EPO和每天一次给予0.9%生理盐水，第3组每天两次给予25 IU/kg EPO。所有给药均通过24号导管鞘经尿道进行。在随访14天后，取大鼠阴茎进行组织病理学评估，以研究固有层中的炎症、海绵体纤维化和血管充血情况。
组织病理学分析显示，接受高剂量EPO的第3组纤维化和炎症较少，血管充血较多。与对照组相比，第2组和第3组的海绵体纤维化和炎症均显著减少，充血增加（P均=0.001）。在第2组和第3组的比较中，这3个参数在统计学上无显著差异（P分别为0.5、0.6、0.27）。
本研究结果表明，EPO在大鼠尿道损伤模型中对海绵体纤维化有预防作用，并能改善尿道伤口愈合。
