重组人肿瘤坏死因子对人鼻咽癌细胞系的体外作用。

Effect of recombinant human tumor necrosis factor on human nasopharyngeal carcinoma cell line in vitro.

作者信息

Ha D K, Lau W H

机构信息

Institute of Radiology & Oncology, Queen Elizabeth Hospital, H.K. Government, Hong Kong.

出版信息

Cancer Lett. 1988 Aug 15;41(2):217-24. doi: 10.1016/0304-3835(88)90119-x.

DOI:10.1016/0304-3835(88)90119-x

PMID:3401845

Abstract

The cytostatic and cytotoxic effects of recombinant human tumor necrosis factor alpha (rHuTNF alpha) on a cultured nasopharyngeal carcinoma (NPC) cell line were studied. rHuTNF alpha inhibited tritiated thymidine ([3H]TdR) and [14C]leucine incorporation by NPC cells. rHuTNF alpha also inhibited the growth of NPC cells as determined by microphotometry. Cytotoxic effect of rHuTNF alpha was observed 2 days after incubation. Pulse hyperthermia or continuous hyperthermia in combination with rHuTNF alpha treatment was markedly cytotoxic for NPC cells in vitro. Addition of actinomycin D, cycloheximide, puromycin or vincristine sulfate to NPC cells also potentiated the cytotoxic effect of rHuTNF alpha.

摘要

研究了重组人肿瘤坏死因子α（rHuTNFα）对一种培养的鼻咽癌细胞系的细胞生长抑制和细胞毒作用。rHuTNFα抑制鼻咽癌细胞掺入氚标记胸腺嘧啶核苷（[3H]TdR）和[14C]亮氨酸。通过显微光度测定法确定，rHuTNFα也抑制鼻咽癌细胞的生长。孵育2天后观察到rHuTNFα的细胞毒作用。脉冲热疗或连续热疗联合rHuTNFα处理对体外培养的鼻咽癌细胞具有显著的细胞毒性。向鼻咽癌细胞中添加放线菌素D、环己酰亚胺、嘌呤霉素或硫酸长春新碱也可增强rHuTNFα的细胞毒作用。