Department of Pathology, Federal University of Sao Paulo, UNIFESP, São Paulo, SP, Brazil.
Department of Marine Sciences, Federal University of Sao Paulo, UNIFESP, Santos, SP, Brazil.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2021;56(5):525-536. doi: 10.1080/10934529.2021.1892016. Epub 2021 May 21.
The aim of this study was to evaluate cytogenotoxicity in mammalian cells induced by ingestion of superficial water from SESS. For this purpose, surface water was collected from two points of SESS: São Vicente Channel (SVC) and Piaçaguera Channel (PIC). Four groups (n = 5) of adult male Wistar (8 weeks old) received for 5 days: (a) filtered tap water (water control), (b) tap water with 2.4% of NaCl (saline control), (c) estuarine water from PIC and (d) estuarine water from SVC. Results demonstrated that Ki67 immunoexpression was higher in hepatocytes exposed to both sampling site, while demonstrated downregulation in rat liver exposed to estuarine water. There was also significant increase in micronuclei frequency in bone marrow cells and hepatocytes, and DNA damage in blood and liver of rats exposed to estuarine water from SVC and PIC. In summary, studies with complex mixtures, such as contaminated estuarine water are important since this work confirmed by experiments using mammalian cells of rats that SESS water are genotoxic, mutagenic and cytotoxic, denoting concern for environmental health.
本研究旨在评估哺乳动物细胞因摄入 SESS 地表水而产生的细胞遗传毒性。为此，从 SESS 的两个点收集了地表水：圣文森特海峡（SVC）和皮亚西加雷亚海峡（PIC）。4 组（n = 5）成年雄性 Wistar 大鼠（8 周龄）连续 5 天接受以下处理：（a）过滤自来水（水对照）、（b）含 2.4%NaCl 的自来水（盐对照）、（c）PIC 的河口水和（d）SVC 的河口水。结果表明，暴露于两个采样点的肝细胞中 Ki67 免疫表达更高，而暴露于河口水的大鼠肝脏中则下调。暴露于 SVC 和 PIC 河口水的大鼠骨髓细胞和肝细胞中的微核频率以及血液和肝脏中的 DNA 损伤均显著增加。总之，对于复杂混合物的研究很重要，如受污染的河口水。本研究使用大鼠的哺乳动物细胞进行实验，证实了 SESS 水具有遗传毒性、致突变性和细胞毒性，这表明对环境健康的关注。
