人乳头瘤病毒 16 型在口腔鳞状细胞癌中的定位和特征。
Localization and characterization of human papillomavirus-16 in oral squamous cell carcinoma.
机构信息
Oral Diagnostic Sciences Department, College of Dentistry, University of Florida, Gainesville, FL, USA.
Oral Medicine and Periodontology Department, Faculty of Dentistry, Mansoura University, Mansoura, FL, USA.
出版信息
Oral Dis. 2023 Mar;29(2):436-444. doi: 10.1111/odi.13920. Epub 2021 Jun 21.
OBJECTIVES
The role of Human papillomavirus (HPV) in the oral squamous cell carcinoma (OSCC) has not been completely elucidated. The purpose of the present study was to investigate the prevalence and localization of HPV-16 virus in OSCC and to correlate HPV-16 positivity and p16INK4A expression with the clinical and pathological features of OSCC.
METHODS
The archives of Oral Pathology at the University of Florida, College of Dentistry were accessed for demographic, clinical, histopathological data, and slides of 114 OSCC patients. HPV-16 positivity of OSCC was evaluated by p16INK4A immunohistochemistry (IHC) and HPV-16 E6/E7mRNA by in situ hybridization (ISH).
RESULTS
Out of 114 consecutive pathological slides of OSCC, 16 samples (14%) showed positivity for p16INK4A by IHC and 14 samples (12%) were positive for HPV-16 E6/E7mRNA ISH and the Positivity showed a significant correlation with the patients' age, alcohol consumption, and the degree of OSSC differentiation. The hard palate showed the highest positivity of p16INK4A IHC and HPV-16 mRNA ISH (38%, 36% respectively).
CONCLUSION
HPV-16 is a significant factor in oral carcinogenesis. We recommend using p16INK4A as a surrogate marker for HPV detection in OSCC, which can be complemented by RNA ISH for the identification of HPV subtypes.
目的
人乳头瘤病毒（HPV）在口腔鳞状细胞癌（OSCC）中的作用尚未完全阐明。本研究的目的是调查 HPV-16 病毒在 OSCC 中的流行率和定位，并将 HPV-16 阳性率和 p16INK4A 表达与 OSCC 的临床和病理特征相关联。
方法
访问佛罗里达大学牙科学院口腔病理学档案，获取 114 例 OSCC 患者的人口统计学、临床、组织病理学数据和幻灯片。通过 p16INK4A 免疫组化（IHC）和 HPV-16 E6/E7mRNA 原位杂交（ISH）评估 OSCC 的 HPV-16 阳性率。
结果
在 114 例连续的 OSCC 病理切片中，16 例（14%）通过 IHC 显示 p16INK4A 阳性，14 例（12%）通过 HPV-16 E6/E7mRNA ISH 阳性，阳性率与患者年龄、饮酒量和 OSSC 分化程度显著相关。硬腭显示出最高的 p16INK4A IHC 和 HPV-16 mRNA ISH 阳性率（分别为 38%和 36%）。
结论
HPV-16 是口腔癌发生的重要因素。我们建议使用 p16INK4A 作为 OSCC 中 HPV 检测的替代标志物，通过 RNA ISH 可补充 HPV 亚型的鉴定。
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