Götz Carolin, Drecoll Enken, Straub Melanie, Bissinger Oliver, Wolff Klaus-Dietrich, Kolk Andreas
Department of Maxillofacial and Oral Surgery, Technical University Munich, 81675 München, Germany.
Department of Pathology, Technical University Munich, 81675 München, Germany.
Oncotarget. 2016 Nov 22;7(47):76704-76712. doi: 10.18632/oncotarget.12501.
Head and neck squamous cell carcinomas (HNSCC) are often divided by their aetiology. Noxae associated collectives are compared with the human papilloma virus (HPV)-associated group, whereas different localisations of oral (OSCC) and oropharyngeal (OPSCC) squamous cell carcinomas are mostly discussed as one single group. Our aim was to show that classification by aetiology is not appropriate for OSCC.
HPV DNA was detected by PCR in 7 (3.47%) patients, and we identified 12 (5.94%) positive (+) cases by p16INK4a immunostaining. Only 4 (1.98%) of the p16INK4a+ cases were + for HPV using PCR. Our homogenous collective of OSCC allowed us to compare HPV+ and HPV negative (-) patients without creating bias for tumour localisation, age, gender or tumour stage.
After testing OSCC samples for HPV positivity, we compared the results of two commonly used HPV detection methods, p16INK4a immunostaining and HPV DNA-related PCR, on 202 OSCC patients. HPV subtypes were determined with an HPV LCD Array Kit. Clinicopathological features of the patients were analysed, and the disease specific survival rates (DSS) for HPV+ and HPV- patients were obtained.
p16INK4a immunostaining is a not a reliable HPV detection method for OSCC. Positive p16INK4a immunostaining did not agree with + results from PCR of HPV DNA. Furthermore, the influence of HPV-related oncogenic transformation in OSCC is overestimated. The significance of HPV infection remains clinically unclear, and its influence on survival rates is not relevant to OSCC cases.
头颈部鳞状细胞癌（HNSCC）常根据其病因进行分类。将与有害因素相关的群体与人类乳头瘤病毒（HPV）相关群体进行比较，而口腔鳞状细胞癌（OSCC）和口咽鳞状细胞癌（OPSCC）的不同部位大多作为一个单一群体进行讨论。我们的目的是表明按病因分类不适用于OSCC。
通过PCR在7例（3.47%）患者中检测到HPV DNA，通过p16INK4a免疫染色鉴定出12例（5.94%）阳性（+）病例。在p16INK4a阳性病例中，仅4例（1.98%）通过PCR检测出HPV阳性。我们同质的OSCC群体使我们能够比较HPV阳性（+）和HPV阴性（-）患者，而不会因肿瘤部位、年龄、性别或肿瘤分期产生偏差。
在检测OSCC样本的HPV阳性后，我们比较了202例OSCC患者两种常用的HPV检测方法，即p16INK4a免疫染色和HPV DNA相关PCR的结果。使用HPV LCD Array试剂盒确定HPV亚型。分析患者的临床病理特征，并获得HPV阳性和阴性患者的疾病特异性生存率（DSS）。
p16INK4a免疫染色不是OSCC可靠的HPV检测方法。p16INK4a免疫染色阳性与HPV DNA PCR阳性结果不一致。此外，HPV相关致癌转化在OSCC中的影响被高估。HPV感染的意义在临床上仍不明确，其对生存率的影响与OSCC病例无关。
