Seyed Alian Reyhaneh, Dziewięcka Marta, Kędziorski Andrzej, Majchrzycki Łukasz, Augustyniak Maria
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, Poland.
Centre of Advanced Technology, Adam Mickiewicz University in Poznan, ul. Uniwersytetu Poznańskiego 10, 61-614 Poznań, Poland.
Sci Total Environ. 2021 Sep 20;788:147801. doi: 10.1016/j.scitotenv.2021.147801. Epub 2021 May 15.
This study aimed to identify the physiological responses of house cricket females following short-term exposure to relatively low dietary doses of graphene oxide (GO, 20 μg · g food), silver (Ag, 400 μg · g food) nanoparticles (NPs), or graphene oxide‑silver nanoparticle composite (GO-AgNPs, 20: 400 μg · g food). Energy intake and distribution were measured on the third, sixth, and tenth day. A semi-quantitative API®ZYM assay of digestive enzyme fingerprints was performed on the third and tenth day of continuous treatment. Physicochemical properties of the NPs were obtained by combining SEM, EDX spectrometry, AFM, and DLS techniques. The obtained results showed decreased energy consumption, particularly assimilation as an early response to dietary NPs followed by compensatory changes in feeding activity leading to the same consumption and assimilation throughout the experimental period (10 days). The increased activities of digestive enzymes in NP-treated females compared to the control on the third day of the experiment suggest the onset of compensatory reactions of the day. Moreover, the insects treated with GO-AgNP composite retained more body water, suggesting increased uptake. The observed changes in the measured physiological parameters after exposure to NPs are discussed in light of hormesis.
本研究旨在确定家蟋蟀雌性个体在短期暴露于相对低剂量的氧化石墨烯（GO，20 μg·g食物）、银（Ag，400 μg·g食物）纳米颗粒（NPs）或氧化石墨烯-银纳米颗粒复合物（GO-AgNPs，20:400 μg·g食物）后的生理反应。在第3天、第6天和第10天测量能量摄入和分配情况。在连续处理的第3天和第10天，对消化酶指纹图谱进行了半定量API®ZYM分析。通过结合扫描电子显微镜（SEM）、能量散射X射线光谱法（EDX）、原子力显微镜（AFM）和动态光散射（DLS）技术获得纳米颗粒的物理化学性质。所得结果显示能量消耗减少，尤其是同化作用，这是对饮食中纳米颗粒的早期反应，随后进食活动发生补偿性变化，导致在整个实验期（10天）内能量消耗和同化作用相同。与对照组相比，在实验第3天经纳米颗粒处理的雌性个体中消化酶活性增加，表明当天出现了补偿反应。此外，用GO-AgNP复合物处理的昆虫保留了更多的身体水分，表明摄取量增加。根据兴奋效应讨论了暴露于纳米颗粒后所观察到的测量生理参数的变化。
