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暴露于氧化石墨烯-银纳米粒子复合物的家蟋蟀（昆虫纲：直翅目）中消化功能的浓度依赖性紊乱

Concentration-dependent disturbances of digestive functions in house cricket (Insecta: Orthoptera) exposed to GO-AgNP composite.

作者信息

Alian Reyhaneh Seyed, Flasz Barbara, Kędziorski Andrzej, Rost-Roszkowska Magdalena, Rozpędek Katarzyna, Majchrzycki Łukasz, Augustyniak Maria

机构信息

Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, 40-007, Katowice, Poland.

Institute of Physics, Faculty of Materials Engineering and Technical Physics, Poznan University of Technology, Piotrowo 3, 60-965, Poznan, Poland.

出版信息

Sci Rep. 2025 Apr 13;15(1):12699. doi: 10.1038/s41598-025-97589-w.

DOI:10.1038/s41598-025-97589-w
PMID:40223030
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11994775/
Abstract

This study investigates the effects of graphene oxide (GO) and silver nanoparticle (AgNP) composite (GO-AgNP) on the digestive physiology and gut ultrastructure of Acheta domesticus (house cricket) during extended exposure. Various concentrations of GO-AgNP were tested to assess their impact on food consumption, assimilation, cell status (Dead Cells and ROS + cells), gut enzyme activity, and structural damage to gut cells. Concentration and exposure time had significant effects on oxidative stress, enzyme activity, and gut cell structure. The applied composite reduced cumulative food consumption and assimilation efficiency. Enzyme assays showed that lower concentrations enhanced carbohydrate-degrading enzyme activity, while higher concentrations inhibited protease activity. Histological analysis revealed structural damage to gut epithelial cells and signs of autophagy or necrosis at higher concentrations. These results suggest that GO and AgNPs contribute to oxidative stress, cell cycle disruption, and apoptosis, with AgNPs having a potentially stronger effect than GO. The disturbed enzyme activity may result from conformational changes caused by nanoparticle agglomeration. These findings underline potential risks associated with the environmental or agricultural use of GO-AgNP composites.

摘要

本研究调查了氧化石墨烯（GO）和银纳米颗粒（AgNP）复合材料（GO-AgNP）在长期暴露期间对家蟋蟀消化生理和肠道超微结构的影响。测试了不同浓度的GO-AgNP，以评估它们对食物消耗、同化、细胞状态（死细胞和ROS+细胞）、肠道酶活性以及肠道细胞结构损伤的影响。浓度和暴露时间对氧化应激、酶活性和肠道细胞结构有显著影响。所应用的复合材料降低了累积食物消耗和同化效率。酶分析表明，较低浓度增强了碳水化合物降解酶的活性，而较高浓度抑制了蛋白酶活性。组织学分析显示肠道上皮细胞结构受损，且在较高浓度下有自噬或坏死迹象。这些结果表明，GO和AgNP会导致氧化应激、细胞周期紊乱和细胞凋亡，其中AgNP的影响可能比GO更强。酶活性受到干扰可能是由于纳米颗粒团聚引起的构象变化所致。这些发现强调了与GO-AgNP复合材料在环境或农业应用相关的潜在风险。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/307fd9b505e6/41598_2025_97589_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/629ba880f184/41598_2025_97589_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/08448fe3b975/41598_2025_97589_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/aff058883cec/41598_2025_97589_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/3dd4d4fddae9/41598_2025_97589_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/882cd9485072/41598_2025_97589_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/2c7db9c24a48/41598_2025_97589_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/479676b73c1c/41598_2025_97589_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/307fd9b505e6/41598_2025_97589_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/629ba880f184/41598_2025_97589_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/2ce4cd3ad3e5/41598_2025_97589_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/08448fe3b975/41598_2025_97589_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/aff058883cec/41598_2025_97589_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/3dd4d4fddae9/41598_2025_97589_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/882cd9485072/41598_2025_97589_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/2c7db9c24a48/41598_2025_97589_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/479676b73c1c/41598_2025_97589_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b85/11994775/307fd9b505e6/41598_2025_97589_Fig9_HTML.jpg

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本文引用的文献

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Nanoscale. 2024 Dec 19;17(1):540-551. doi: 10.1039/d4nr03333g.
2
Insect Models to Study Human Lipid Metabolism Disorders.用于研究人类脂质代谢紊乱的昆虫模型
Adv Exp Med Biol. 2024 Oct 16. doi: 10.1007/5584_2024_827.
3
Immobilization of silver-loaded graphene oxide (Ag-GO) on canvas fabric support for catalytic conversion of 4 nitrophenol.
载银氧化石墨烯（Ag-GO）在帆布织物上的固定化及其对 4-硝基苯酚催化转化的研究。
Environ Sci Pollut Res Int. 2024 Aug;31(39):51815-51833. doi: 10.1007/s11356-024-34586-y. Epub 2024 Aug 11.
4
Carbon materials and their metal composites for biomedical applications: A short review.用于生物医学应用的碳材料及其金属复合材料：简短综述。
Nanoscale. 2024 Sep 12;16(35):16313-16328. doi: 10.1039/d4nr02059f.
5
Cellular response during cellular starvation: A battle for cellular survivability.细胞饥饿时的细胞反应：一场关乎细胞生存能力的战斗。
Cell Biochem Funct. 2024 Jul;42(5):e4101. doi: 10.1002/cbf.4101.
6
Nano-enabled smart and functional materials toward human well-being and sustainable developments.用于人类福祉和可持续发展的纳米智能功能材料。
Nanotechnology. 2024 Jun 14;35(35). doi: 10.1088/1361-6528/ad4dac.
7
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Insects. 2024 Jan 29;15(2):89. doi: 10.3390/insects15020089.
8
Nanotechnology in precision agriculture: Advancing towards sustainable crop production.精准农业中的纳米技术：迈向可持续作物生产
Plant Physiol Biochem. 2024 Jan;206:108244. doi: 10.1016/j.plaphy.2023.108244. Epub 2023 Nov 30.
9
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J Hazard Mater. 2024 Jan 5;461:132623. doi: 10.1016/j.jhazmat.2023.132623. Epub 2023 Sep 23.
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Appl Biochem Biotechnol. 2024 Jun;196(6):3636-3669. doi: 10.1007/s12010-023-04719-z. Epub 2023 Sep 5.