School of Chemistry and Chemical Engineering, Southeast University, Nanjing 214122, PR China.
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 214122, PR China.
Colloids Surf B Biointerfaces. 2021 Sep;205:111854. doi: 10.1016/j.colsurfb.2021.111854. Epub 2021 May 17.
Nanoparticles (NPs) were hypothesized to enhance fermentation processes and assist microorganisms in producing valuable biopolymers. Donors of trace iron, i.e., FeSO·7HO, zero-valence iron nanoparticles (Fe NPs), and ferric oxide nanoparticles (α-FeO NPs), were tested to study the impact on hyaluronic acid (HA) production. The bioprocess with the addition of 30 mg/L Fe NPs produced higher HA than the other groups. However, Fe NPs were limited by the synergistic effect of geomagnetism and high surface energy, resulting in obvious agglomeration behavior. To address this, we developed novel sucrose-modified iron nanoparticles (SM-Fe NPs), which showed effective improvement of dispersion and agglomeration. Concerning the SM-Fe NP additives, an adequate supply of nutrients and trace elements provided sufficient substrates and energy for the reproduction of Streptococcus zooepidemicus. Furthermore, the highest HA production with the addition of 30 mg/L SM-Fe NPs was 0.226 g/L, and the dry weight of the produced HA increased 3.28 times compared with the control group (0.069 g/L). This work significantly improved HA production and presented promising opportunities for industrial production.
纳米颗粒（NPs）被假设可以增强发酵过程，并帮助微生物生产有价值的生物聚合物。本研究测试了痕量铁供体，即 FeSO·7HO、零价铁纳米颗粒（Fe NPs）和氧化铁纳米颗粒（α-FeO NPs），以研究它们对透明质酸（HA）生产的影响。添加 30mg/L Fe NPs 的生物工艺产生的 HA 高于其他组。然而，Fe NPs 受到地磁场和高表面能的协同作用的限制，导致明显的团聚行为。为了解决这个问题，我们开发了新型蔗糖修饰的铁纳米颗粒（SM-Fe NPs），显示出有效的分散和团聚改善效果。关于 SM-Fe NP 添加剂，充足的营养和微量元素供应为链球菌的繁殖提供了足够的底物和能量。此外，添加 30mg/L SM-Fe NPs 时 HA 的最高产量为 0.226g/L，与对照组（0.069g/L）相比，产生的 HA 的干重增加了 3.28 倍。这项工作显著提高了 HA 的产量，为工业生产提供了有前景的机会。
