National Institute of Laser Enhanced Sciences, Cairo University, 12613 Giza, Egypt.
Department of Biochemistry, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt; Faculty of Science, Department of Chemistry, Taif University, Taif 21974, Saudi Arabia.
Carbohydr Polym. 2018 Jul 15;192:135-142. doi: 10.1016/j.carbpol.2018.03.037. Epub 2018 Mar 19.
This work aims at developing a novel hyaluronic acid (HA) production method using magnetic nanoparticles (NPs). In a separate process, HA was produced with the addition of the amino acids (AA) as bio-additives. Regarding the NPs additives, the results showed that the highest dry weight of the produced HA was 0.264 g/l with the addition of 20 mg/l of FeO NPs. Concerning the AA additives, the results showed that the highest dry weight of the produced HA was 0.079 g/l with the addition of 0.26 g/l of glutamic acid (GA) compared to the control produced 0.065 g/l. These results led to further develop a novel HA production method which is preparing the FeO NPs using GA as stabilizer, where the results showed that dry weight of the produced HA was 0.435 g/l with the addition of 20 mg/l of FeO-GA NPs.
本工作旨在开发一种使用磁性纳米粒子 (NPs) 的新型透明质酸 (HA) 生产方法。在另一个过程中，添加氨基酸 (AA) 作为生物添加剂来生产 HA。关于 NPs 添加剂，结果表明，添加 20mg/L 的 FeO NPs 时，所产生的 HA 的干重最高为 0.264g/L。关于 AA 添加剂，结果表明，添加 0.26g/L 的谷氨酸 (GA) 时，所产生的 HA 的干重最高为 0.079g/L，与对照相比（产生 0.065g/L）。这些结果促使我们进一步开发了一种新型的 HA 生产方法，即使用 GA 作为稳定剂制备 FeO NPs，结果表明，添加 20mg/L 的 FeO-GA NPs 时，所产生的 HA 的干重为 0.435g/L。
