Wang D S, Li S W, Zeng C Q, Cheng R X, Xue S B
Department of Biology, Beijing Normal University, China.
Cytometry. 1988 Jul;9(4):368-73. doi: 10.1002/cyto.990090414.
Using DAPI, rabbit antitubulin antibody, FITC-labeled goat anti-rabbit IgG, and TRITC-phalloidin to stain individual cells, the microspectrophotometric analysis showed that three markers that represent the nucleus, microtubules (MT), and microfilaments (MF), respectively, could be recognized in individual cells without interference. The phase of the cell cycle was determined by DNA content. We found that in Indian muntjac (IM) cells, the amount of tubulin in G2 and M phases was about twice as much as that in G1 phase. In G2 cells, the cytoplasmic microtubule complex (CMTC) became denser than in G1 cells. The cytoplasmic MT extent in basically the same orientation as MF bundles in interphase. The regions where the MT is denser also have a denser MF distribution.
使用4',6-二脒基-2-苯基吲哚（DAPI）、兔抗微管蛋白抗体、异硫氰酸荧光素（FITC）标记的山羊抗兔免疫球蛋白G（IgG）以及四甲基异硫氰酸罗丹明（TRITC）-鬼笔环肽对单个细胞进行染色，显微分光光度分析表明，分别代表细胞核、微管（MT）和微丝（MF）的三种标记物能够在单个细胞中被识别且互不干扰。细胞周期阶段由DNA含量确定。我们发现，在印度麂（IM）细胞中，G2期和M期的微管蛋白量约为G1期的两倍。在G2期细胞中，细胞质微管复合体（CMTC）比G1期细胞中的更密集。间期细胞质MT的延伸方向与MF束基本相同。MT更密集的区域MF分布也更密集。
