Tanaka K, Nanbara S, Tanaka T, Koide H, Hayashi T
Department of Clinical Pathology, Osaka Medical College, Japan.
Diabetes Res Clin Pract. 1988 May 19;5(1):71-5. doi: 10.1016/s0168-8227(88)80081-0.
Enzyme activity in the livers of mice was studied in examining the metabolic disturbances of diabetes. Spontaneously non-obese diabetic (NOD) mice, mice with alloxan-induced diabetes (Allo), and control ICR mice were used. As NOD mice undergo a spontaneous pathogenic process over time, younger and older NOD mice were compared (non-diabetic and diabetic) as were control ICR mice. Two liver enzymes became more active with age, aspartate aminotransferase (AST) and alanine aminotransferase (ALT). AST activity increased more in the hyperglycemic mice, i.e., the diabetic NOD and the Allo mice, than in the normoglycemic group, i.e., the ICR and non-diabetic NOD mice. Abnormally high AST activity was seen in the cytosolic fraction of the liver but not in the mitochondrial fraction. The changes in enzyme activity in diabetic mice were independent of any age-associated changes. The higher AST levels in diabetic mice are thought to be consistent with their greater need for gluconeogenic substrate. AST showed a more notably higher increase than did ALT in this study, and lactate dehydrogenase showed no significant changes.
在研究糖尿病的代谢紊乱过程中，对小鼠肝脏中的酶活性进行了研究。使用了自发性非肥胖糖尿病（NOD）小鼠、四氧嘧啶诱导糖尿病小鼠（Allo）以及对照ICR小鼠。由于NOD小鼠会随着时间推移经历自发的致病过程，因此比较了年轻和年老的NOD小鼠（非糖尿病和糖尿病状态）以及对照ICR小鼠。两种肝脏酶随着年龄增长活性增强，即天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）。与血糖正常组（即ICR和非糖尿病NOD小鼠）相比，高血糖小鼠（即糖尿病NOD和Allo小鼠）中AST活性增加得更多。在肝脏的胞质部分观察到异常高的AST活性，但在线粒体部分未观察到。糖尿病小鼠中酶活性的变化与任何与年龄相关的变化无关。糖尿病小鼠中较高的AST水平被认为与其对糖异生底物的更大需求一致。在本研究中，AST的升高比ALT更为显著，而乳酸脱氢酶没有显著变化。
