Luo Liru, Fan Wei, Qin Jingping, Guo Shiyin, Xiao Hang, Tang Zhonghai
College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Hunan Engineering Technology Research Center for Rapeseed Oil Nutrition Health and Deep Development, Changsha 410128, China.
Curr Issues Mol Biol. 2023 Jun 29;45(7):5403-5421. doi: 10.3390/cimb45070343.
This study investigated the pharmacological and pathological effects of aqueous mulberry leaf extract on type 1 diabetes mellitus mice induced with an intraperitoneal injection of streptozotocin (STZ). Diabetic mice were randomized into six groups: control (normal group), model, metformin-treated mice, and high-dose, medium-dose, and low-dose mulberry. The mulberry-treated mice were divided into high-, medium-, and low-dose groups based on the various doses of aqueous mulberry leaf extract during gavage. The efficacy of the six-week intervention was evaluated by measuring levels of fasting plasma glucose, alkaline phosphatase, alanine aminotransferase, aspartate transaminase, blood urea nitrogen, gamma-glutamyl transferase, glucose, high-density lipoprotein cholesterol, lactate dehydrogenase, and low-density lipoprotein cholesterol and recording body weight. Results revealed that mulberry leaf extract exhibited an ideal hypoglycemic effect, and the high-dose group was the most affected. Histology analysis, glycogen staining and apoptosis detection were used to study the extract's effects on the liver, kidney, and pancreatic cells of diabetic mice, enabling the assessment of its effectiveness and complications on a clinical and theoretical basis. It was shown that a certain concentration of aqueous mulberry leaf extract repaired the islet cells of type 1 diabetes mellitus mice, promoting normal insulin secretion. Herein, it was confirmed that mulberry leaf could be used to develop new hypoglycemic drugs or functional health food with broad applicability.
本研究探讨了腹腔注射链脲佐菌素（STZ）诱导的1型糖尿病小鼠经桑叶水提取物处理后的药理和病理作用。将糖尿病小鼠随机分为六组：对照组（正常组）、模型组、二甲双胍治疗组以及高剂量、中剂量和低剂量桑叶组。根据灌胃期间桑叶水提取物的不同剂量，将接受桑叶处理的小鼠分为高、中、低剂量组。通过测量空腹血糖、碱性磷酸酶、谷丙转氨酶、谷草转氨酶、血尿素氮、γ-谷氨酰转移酶、血糖、高密度脂蛋白胆固醇、乳酸脱氢酶和低密度脂蛋白胆固醇水平并记录体重，评估为期六周干预的效果。结果显示，桑叶提取物呈现出理想的降血糖作用，其中高剂量组受影响最大。采用组织学分析、糖原染色和凋亡检测研究该提取物对糖尿病小鼠肝脏、肾脏和胰腺细胞的影响，以便在临床和理论基础上评估其有效性和并发症。结果表明，一定浓度的桑叶水提取物修复了1型糖尿病小鼠的胰岛细胞，促进了正常胰岛素分泌。在此证实，桑叶可用于开发具有广泛适用性的新型降血糖药物或功能性保健食品。
