Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, 230026, China.
Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, 230031, China.
Anal Sci. 2021 Dec 10;37(12):1695-1700. doi: 10.2116/analsci.21P098. Epub 2021 May 21.
Alpha-fetoprotein (AFP) is an important disease biomarker, relating to cancers such as hepatocarcinomas and gastric cancer. However, traditional methods are time-consuming, relied on bulky instruments and trained professionals, cannot satisfy the demand for low cost and point-of-care testing (POCT). In this study, a power-free POCT device was developed for the rapid and low-cost detection of AFP via one-sampling. Based on the principle of sandwich immunofluorescence, the chip is capable of automatically accomplishing on-chip mixing, labeling and capturing procedures, which only require that operator add 40 μL sample into the chip one time. The proposed device is capable of sensitively detecting human AFP in FBS with a dynamic range of 10 - 1000 ng/mL and LOD (1.88 ng/mL) within a short time of 3 min. Predictably, our method holds a great potential to be applied in the POC diagnostics of proteins, especially for some regions that are resource-limited.
甲胎蛋白（AFP）是一种重要的疾病生物标志物，与肝癌和胃癌等癌症有关。然而，传统方法耗时耗力，依赖于大型仪器和专业人员，无法满足低成本和即时检测（POCT）的需求。在本研究中，开发了一种无电源 POCT 设备，用于通过单次采样快速、低成本地检测 AFP。基于夹心免疫荧光原理，该芯片能够自动完成芯片内混合、标记和捕获步骤，仅需操作人员将 40 μL 样本一次加入芯片。该设备能够在短时间内（3 分钟内）灵敏地检测到 FBS 中的人 AFP，检测范围为 10-1000ng/mL，检测限（LOD）为 1.88ng/mL。可以预见，我们的方法在蛋白质的 POCT 诊断中具有很大的应用潜力，特别是对于一些资源有限的地区。
