Department of Chemistry, Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China.
Shanghai Suxin Biotechnology Co. Ltd, and IgeneTec Diagnostic Products Co. Ltd., Shanghai, 201318, PR China.
Anal Chim Acta. 2020 Jun 29;1118:18-25. doi: 10.1016/j.aca.2020.04.031. Epub 2020 Apr 13.
Simplifying the procedure of immunoassay is still a challenge due to problems such as multiple washing processes, complicated chemical modification and expensive cost. In this study, we developed a portable centrifugal microchip fluorescence immunoassay for washing-free, rapid, quantitative and point-of-care (POC) detection of protein. The designed microchip was fabricated by polycarbonate and assembled by double-sided adhesive tape using injecting molding with high scalability and low cost. The centrifugal strategy is capable of washing-out the bio-fluid and improving signal-to-noise ratio. Matrix nano-spotting method was employed to facilitate satisfactory immunological binding sites with the advantage of high capture efficiency and reproducibility. The proposed approach was capable of sensitively detecting procalcitonin (PCT) with a wide dynamic ranging from 0.10 ng/mL to 70.00 ng/mL within 10 min. Furthermore, this novel integrated diagnostic tool was successfully applied to detect PCT in 101 clinical samples with good consistency with Roche's method, indicating its attractive practical application capability. With favorable simplicity, rapidity, low cost and excellent analytical performance, our method holds great promise for POC diagnostics of proteins.
由于多次洗涤过程、复杂的化学修饰和昂贵的成本等问题，简化免疫分析的程序仍然是一个挑战。在这项研究中，我们开发了一种便携式离心微流控芯片荧光免疫分析方法，用于无洗涤、快速、定量和即时检测（POC）蛋白质。设计的微芯片由聚碳酸酯制成，并使用双面胶带通过注塑成型组装，具有高可扩展性和低成本。离心策略能够冲洗生物流体并提高信噪比。基质纳米点样方法被用来促进与高捕获效率和重现性的优势令人满意的免疫结合位点。所提出的方法能够在 10 分钟内灵敏地检测降钙素原（PCT），具有从 0.10ng/mL 到 70.00ng/mL 的宽动态范围。此外，这种新型集成诊断工具成功地用于检测 101 个临床样本中的 PCT，与罗氏方法具有良好的一致性，表明其具有吸引人的实际应用能力。由于具有良好的简单性、快速性、低成本和优异的分析性能，我们的方法有望用于 POC 诊断蛋白质。
