Taira Shu, Kiriake-Yoshinaga Aya, Shikano Hitomi, Ikeda Ryuzoh, Kobayashi Shoko, Yoshinaga Kazuaki
Faculty of Food and Agricultural Sciences Fukushima University Fukushima Japan.
Research Center for Food Safety Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan.
Food Sci Nutr. 2021 Mar 30;9(5):2779-2784. doi: 10.1002/fsn3.2232. eCollection 2021 May.
The localization of essential oils, including flavor components, in perilla herb () were visually determined using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) imaging. The surface of a perilla leaf was peeled using a cyanoacrylate adhesion compound and contained oil glands that retained their morphology and chemical properties. We imaged the three essential oils perillaldehyde, β-caryophyllene, and rosmarinic acid (RA). Perillaldehyde was derivatized using glycine to prevent evaporation and allow its detection and imaging while localized in oil glands. β-caryophyllene also localized in the oil glands and not in the epidermis region. RA was detected throughout the leaf, including the oil glands. Quantitative data for the three essential oils were obtained by gas chromatography- or liquid chromatography-MS. The concentrations of perillaldehyde, β-caryophyllene, and RA were 12.6 ± 0.62, 0.27 ± 0.02, and 0.16 ± 0.02 [mg/g] in the paste sample of perilla herb. Peeling using a cyanoacrylate adhesion compound, and derivatization of a target such as an aroma component have great potential for mass spectrometry imaging for multiple essential oils.
使用基质辅助激光解吸/电离（MALDI）质谱成像技术，直观地确定了紫苏叶中精油（包括风味成分）的定位。使用氰基丙烯酸酯粘附剂剥离紫苏叶表面，叶表面含有保持其形态和化学性质的油腺。我们对紫苏醛、β-石竹烯和迷迭香酸（RA）这三种精油进行了成像。紫苏醛用甘氨酸衍生化，以防止其蒸发，并在其定位在油腺中时进行检测和成像。β-石竹烯也定位在油腺中，而非表皮区域。在整个叶片中都检测到了RA，包括油腺。通过气相色谱或液相色谱-质谱获得了这三种精油的定量数据。在紫苏叶糊剂样品中，紫苏醛、β-石竹烯和RA的浓度分别为12.6±0.62、0.27±0.02和0.16±0.02[mg/g]。使用氰基丙烯酸酯粘附剂进行剥离，以及对诸如香气成分等目标进行衍生化，在多种精油的质谱成像方面具有巨大潜力。
